One-step sandwich enzyme immunoassay for soluble human thrombomodulin using monoclonal antibodies.
One-step sandwich enzyme immunoassay for soluble human thrombomodulin using monoclonal antibodies.
复制标题
使用单克隆抗体对可溶性人血栓调节蛋白进行一步夹心酶免疫分析。
DOI：
10.1016/0009-8981(90)90221-d
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
K. Suzuki
中科院分区：
文献类型：
--
作者：
S. Kodama;E. Uchijima;M. Nagai;K. Mikawatani;T. Hayashi;K. Suzuki
制备了6株抗人血栓调节蛋白（TM）的单克隆抗体。所有人都识别出TM的弹性蛋白酶消化片段，其中包含6个表皮生长因子（EGF）样结构域。我们开发了一个一步夹心酶免疫测定可溶性TM使用2个抗体，其中之一，抑制凝血酶结合TM，固定到聚苯乙烯球，和其他，这并不抑制凝血酶结合，但抑制蛋白C激活辅因子的TM的活性，被用作过氧化物酶标记的共轭物。本试验的灵敏度为可溶性TM 1 μg/l。发现系统性红斑狼疮患者血清中可溶性TM的水平与健康受试者血清中的水平相比显著增加。
Six monoclonal antibodies for human thrombomodulin (TM) were prepared. All of them recognized an elastase-digested fragment of TM which contains 6 epidermal growth factor (EGF)-like structural domains. We developed a one-step sandwich enzyme immunoassay for soluble TM by using 2 antibodies; one of them, which inhibited thrombin-binding to TM, was fixed to polystyrene balls, and the other, which did not inhibit the thrombin-binding, but inhibited the protein C-activating cofactor activity of TM, was used as peroxidase-labeled conjugate. The sensitivity of this assay was 1 μg/l for soluble TM. The level of soluble TM was found to be significantly increased in sera of patients with systemic lupus erythematosus in comparison to the level in sera of healthy subjects.
DOI：
10.1016/s0021-9258(19)69233-1
发表时间：
1981-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
W. Owen;C. Esmon
通讯作者：
W. Owen;C. Esmon
血栓调节蛋白功能域的蛋白水解形成和特性。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kurosawa,S;Galvin,JB;Esmon,NL;Esmon,CT
通讯作者：
Esmon,CT