The importance of analytical techniques in allelopathy studies with the reported allelochemical catechin as an example
The importance of analytical techniques in allelopathy studies with the reported allelochemical catechin as an example
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DOI：
10.1007/s10530-008-9250-1
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发表时间：
2009-02-01
影响因子：
2.9
通讯作者：
Hufbauer, Ruth A.
中科院分区：
文献类型：
--
作者：
Blair, Amy C.;Weston, Leslie A.;Hufbauer, Ruth A.
化感作用的证明可能具有挑战性。开发严格的分析技术来检测和量化土壤或液体介质中感兴趣的化合物，为设计包含候选化感化学物质的生态相关实验奠定了基础。本文讨论了分析技术的基本组成部分，包括方法开发、验证和适当的控制。以对斑点矢车菊候选化感物质儿茶素的研究为例，证明在数据收集和后续出版物中包含这些成分的重要性。这个例子表明，当出版物中不包含有关对照和方法验证的信息时，研究小组之间的对比结果可能很难解释。最近的研究表明，儿茶素不太可能驱动斑矢车菊的入侵，因此对该系统的未来研究应集中于斑矢车菊的替代候选毒素。通过采用适当的分析技术（例如此处概述的技术），可以为以生态为导向的实验奠定坚实的基础，这些实验检查化感物质在构建群落中的作用。
Allelopathy can be challenging to demonstrate. Developing rigorous analytical techniques to detect and quantify compound(s) of interest from soil or liquid media lays the foundation for designing ecologically relevant experiments that incorporate candidate allelochemicals. In this paper, fundamental components of analytical techniques, including method development, validation, and appropriate controls are discussed. Research on the candidate allelochemical from spotted knapweed, catechin, is used as an example to demonstrate the importance of including these components both during data collection and in subsequent publications. This example shows how contrasting results between research groups can be difficult to interpret when information on controls and method validation are not included in publications. Recent research suggests that catechin is not likely driving spotted knapweed's invasion, and thus future research on this system should focus on alternate candidate toxins from spotted knapweed. By employing appropriate analytical techniques, such as those outlined here, a strong foundation can be laid for ecologically oriented experiments that examine the role of allelochemicals in structuring communities.