Differential effects of high-physiological oestrogen on the degeneration of mandibular condylar cartilage and subchondral bone
Differential effects of high-physiological oestrogen on the degeneration of mandibular condylar cartilage and subchondral bone
复制标题
高生理雌激素对下颌髁软骨及软骨下骨退变的差异作用
DOI：
10.1016/j.bone.2018.03.008
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发表时间：
2018-06-01
期刊：
影响因子：
4.1
通讯作者：
Yu, Shibin
中科院分区：
文献类型：
--
作者：
Ye, Tao;Sun, Dongliang;Yu, Shibin
女性颞下颌关节紊乱病（TMD）的发病率较高，尤其是在性腺发育完整的青春期或生育期，提示雌激素在TMD的发生发展中起重要作用，但其发病机制尚不清楚。在这项研究中，单侧前牙反（UAC）被用来创建颞下颌关节骨关节炎（TMJ OA）模型大鼠，而17 β-雌二醇（E-2）注射，以模拟高生理水平的雌激素患者。采用显微CT扫描、组织学染色和实时荧光定量PCR方法观察髁突软骨和软骨下骨的退行性改变。结果显示，UAC术后大鼠髁突软骨和软骨下骨发生明显降解，软骨厚度减少，细胞外基质丢失，软骨细胞凋亡增多，促炎因子和分解代谢因子表达增加，骨密度降低，破骨细胞活性增加。补充E-2可加重UAC所致的髁突软骨退化，但可逆转其引起的软骨下骨异常骨吸收。结果表明，高生理性雌激素在TMJOA早期对髁突软骨起破坏作用，对软骨下骨起保护作用。在未来的OA治疗中，应认真考虑这些双重和不同的影响。(C)2018由Elsevier Inc.出版
The striking predilection of temporomandibular disorders (TMD) in women, especially during gonad-intact puberty or reproductive years, indicates that oestrogen plays an important role in the progression of TMD, but the underlying mechanism remains unclear. In this study, unilateral anterior crossbite (UAC) was used to create temporomandibular joint osteoarthritis (TMJ OA) models in rats, while 17 beta-estradiol (E-2) injections were applied to mimic patients with high-physiological levels of oestrogen. Micro-CT scanning, histological staining and real-time PCR assays were preformed to observe the degenerative changes in the mandibular condylar cartilage and subchondral bone. The results showed that obvious degradation was found in the condylar cartilage and subchondral bone of rats with UAC procedure, including decreased cartilage thickness, loss of extracellular matrix, increased apoptotic chondrocytes and expression of pro-inflammatory and catabolic factors, decreased bone mineral density and increased osteoclast activity. E-2 supplements aggravated the condylar cartilage degradation but reversed the abnormal bone resorption in the subchondral bone induced by UAC Our results revealed that high-physiological oestrogen plays a destructive role in condylar cartilage but a protective role in subchondral bone at the early stage of TMJ OA. These dual and distinct effects should be given serious consideration in future OA treatments. (C) 2018 Published by Elsevier Inc.