Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane.
Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane.
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DOI：
10.18632/oncotarget.11315
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发表时间：
2016-09-13
期刊：
影响因子：
--
通讯作者：
Dong W
中科院分区：
文献类型：
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作者：
Du J;Shen J;Wang Y;Pan C;Pang W;Diao H;Dong W
精浆成分对维持精子活力很重要。本研究旨在探讨猪精浆外泌体对精子长期液态保存功能的影响。扫描电镜（SEM）检测显示，猪精浆外泌体具有典型的纳米结构形态，Western blot检测显示外泌体具有AWN、CD 9和CD 63等分子标记。分析了不同比例的猪精浆外泌体对猪精子参数的影响。与不含exosomes的稀释液相比，添加4倍或16倍exosomes的稀释液可提高精子活力，延长有效存活时间，改善精子质膜完整性（p < 0.05），提高总抗氧化能力（T-AOC），降低丙二醛（MDA）含量。与原始精液相比，含有四倍浓度的外泌体的稀释液被确定为抑制过早获能，但不影响体外诱导的获能。对过早获能的抑制可能与外泌体的浓度有关，外泌体已被证明可将包括AWN和PSP-1在内的蛋白质转移到精子中。此外，荧光显微镜和扫描电镜分析表明，稀释液中的exosomes可直接与精子头部膜结合，提高精子质膜的完整性。
Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on sperm parameters of adding different ratio of boar seminal plasma exosomes to boar sperm preparations was analyzed. Compared to the diluent without exosomes, the diluent with four times or sixteen times exosomes compared to original semen had higher sperm motility, prolonged effective survival time, improved sperm plasma membrane integrity (p < 0.05), increased total antioxidant capacity (T-AOC) activity and decreased malondialdehyde (MDA) content. The diluent containing four times concentration of exosomes compared to original semen was determined to inhibit premature capacitation, but not to influence capacitation induced in vitro. Inhibition of premature capacitation is likely related to the concentration of exosomes which had been demonstrated to transfer proteins including AWN and PSP-1 into sperm. In addition, using fluorescence microscopy and scanning electron microscopy analysis, it was demonstrated that exosomes in diluent were directly binding to the membrane of sperm head which could improve sperm plasma membrane integrity.