Timing controllable electrofusion device for aqueous droplet-based microreactors.
Timing controllable electrofusion device for aqueous droplet-based microreactors.
复制标题
DOI：
10.1039/b517178d
复制
发表时间：
2006-05
期刊：
影响因子：
6.1
通讯作者：
Wei-heong Tan;S. Takeuchi
中科院分区：
文献类型：
--
作者：
Wei-heong Tan;S. Takeuchi
本文描述了一种通过施加电场来控制液滴之间的精确融合时刻的电融合装置。该装置允许（i）精确确定化学/生物反应的开始，（ii）反应物与通道壁的最小接触-消除表面吸收问题，（iii）容易制造和（iv）连续观察引发的反应。我们展示了β-半乳糖苷酶和荧光素二-β-D-吡喃半乳糖苷（FDG）液滴的融合，并使用荧光显微镜观察酶促反应。此外，还完成了皮升液滴的顺序融合。
This paper describes an electrofusion device for controlling the precise moment of fusion between droplets by applying an electric field. This device allows (i) accurate determination of the start of chemical/biological reactions, (ii) minimum contact of reactants with channel walls--eliminating surface absorption problems, (iii) easy fabrication and (iv) continuous observation of initiated reaction. We demonstrated the fusion of beta-galactosidase and fluorescein di-beta-D-galactopyranoside (FDG) droplets, and observed the enzymatic reaction using fluorescence microscopy. In addition, sequential fusion of pico-litre droplets was also accomplished.