Support for the prion hypothesis for inheritance of a phenotypic trait in yeast
Support for the prion hypothesis for inheritance of a phenotypic trait in yeast
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DOI：
10.1126/science.273.5275.622
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发表时间：
1996-08-02
期刊：
影响因子：
56.9
通讯作者：
Lindquist, S
中科院分区：
文献类型：
--
作者：
Patino, MM;Liu, JJ;Lindquist, S
通过差速离心分析和Sup35-绿色荧光蛋白融合的显微定位，证实了酵母中一个细胞质遗传的遗传元件[Psi+]是细胞蛋白Sup35的Prionlike聚集体。聚集性依赖于伴侣蛋白Hsp104的细胞内浓度和功能状态，其方式与[PSI+]遗传相同。Sup35的氨基末端和羧基末端结构域导致了[PSI+]的异常行为。[PSI+]改变了新合成的Pron蛋白的构象板条，诱导它们聚集，从而实现了Prion假说的一个主要原则。
A cytoplasmically inherited genetic element in yeast, [PSI+], was confirmed to be a prionlike aggregate of the cellular protein Sup35 by differential centrifugation analysis and microscopic localization of a Sup35-green fluorescent protein fusion. Aggregation depended on the intracellular concentration and functional state of the chaperone protein Hsp104 in the same manner as did [PSI+] inheritance. The amino-terminal and carboxyterminal domains of Sup35 contributed to the unusual behavior of [PSI+]. [PSI+] altered the conformational slate of newly synthesized prion proteins, inducing them to aggregate as well, thus fulfilling a major tenet of the prion hypothesis.