Up-regulation of cerebral cytochrome-c-oxidase and hemodynamics by transcranial infrared laser stimulation: A broadband near-infrared spectroscopy study
Up-regulation of cerebral cytochrome-c-oxidase and hemodynamics by transcranial infrared laser stimulation: A broadband near-infrared spectroscopy study
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DOI：
10.1177/0271678x17691783
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发表时间：
2017-12-01
影响因子：
6.3
通讯作者：
Liu, Hanli
中科院分区：
文献类型：
--
作者：
Wang, Xinlong;Tian, Fenghua;Liu, Hanli
经颅红外激光刺激（TILS）是一种非侵入性的脑光生物学形式。细胞色素c氧化酶（CCO）是线粒体电子传递链的末端酶，被认为是细胞内主要的光受体。我们假设TILS上调脑CCO并引起血流动力学改变。我们提供了1064 nm的激光刺激健康参与者（n = 11）的前额，而宽带近红外光谱被用来获取TILS治疗的皮质区域之前，期间和之后TILS的光反射。还进行了安慰剂实验以进行准确比较。分析了光谱读数的时间过程，并将其拟合为修正的Beer-Lambert定律。相对于安慰剂组，我们观察到（1）氧化CCO的脑浓度显著增加（Delta [CCO]; > 0.08 μ M; p< 0.01），氧合血红蛋白（δ [HbO]; > 0.8 μ M; p< 0.01），和总血红蛋白（Delta [HbT]; > 0.5 μ M; p< 0.01），以及（2）Delta [CCO]与Delta [HbO]之间以及Delta [CCO]与Delta [HbT]之间的线性相互作用。以Delta [CCO]/Delta[HbO]和Delta [CCO]/Delta [HbT]比值作为TILS诱导的代谢-血流动力学耦合指标，量化TILS促进脑代谢和血氧供应之间的耦合强度。本研究首次证明TILS可上调人脑氧化型CCO的表达，并对TILS的生理机制提供了重要的见解。
Transcranial infrared laser stimulation (TILS) is a noninvasive form of brain photobiomulation. Cytochrome-c-oxidase (CCO), the terminal enzyme in the mitochondrial electron transport chain, is hypothesized to be the primary intracellular photoacceptor. We hypothesized that TILS up-regulates cerebral CCO and causes hemodynamic changes. We delivered 1064-nm laser stimulation to the forehead of healthy participants (n = 11), while broadband near-infrared spectroscopy was utilized to acquire light reflectance from the TILS-treated cortical region before, during, and after TILS. Placebo experiments were also performed for accurate comparison. Time course of spectroscopic readings were analyzed and fitted to the modified Beer-Lambert law. With respect to the placebo readings, we observed (1) significant increases in cerebral concentrations of oxidized CCO (Delta [CCO]; > 0.08 mu M; p< 0.01), oxygenated hemoglobin (Delta [HbO]; > 0.8 mu M; p< 0.01), and total hemoglobin (Delta [HbT]; > 0.5 mu M; p< 0.01) during and after TILS, and (2) linear interplays between Delta [CCO] versus Delta [HbO] and between Delta [CCO] versus Delta [HbT]. Ratios of Delta [CCO]/Delta[HbO] and Delta [CCO]/Delta [HbT] were introduced as TILS-induced metabolic-hemodynamic coupling indices to quantify the coupling strength between TILS-enhanced cerebral metabolism and blood oxygen supply. This study provides the first demonstration that TILS causes up-regulation of oxidized CCO in the human brain, and contributes important insight into the physiological mechanisms.