Effects of Linderae radix extracts on a rat model of alcoholic liver injury.
Effects of Linderae radix extracts on a rat model of alcoholic liver injury.
复制标题
乌药提取物对大鼠酒精性肝损伤模型的影响
DOI：
10.3892/etm.2016.3244
复制
发表时间：
2016-06
影响因子：
2.7
通讯作者：
Lou ZH
中科院分区：
文献类型：
--
作者：
Wang JW;Chen XY;Hu PY;Tan MM;Tang XG;Huang MC;Lou ZH
传统的治疗方法对酒精性肝病效果不佳。Linderae radix（LR），Lindera aggregata（西姆斯）Kosterm.的干燥根，在治疗各种疾病的传统中药中经常使用，并且已经显示出对肝损伤的保护作用。本研究采用不同溶剂制备甘草提取物，然后给药于大鼠，建立乙醇诱导的肝损伤模型。本研究旨在探讨枸杞子提取物对急性酒精性肝损伤的治疗作用及其机制。采用全自动生化分析仪测定血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、甘油三酯（TG）、胆固醇（TC）、丙二醛（MDA）和超氧化物歧化酶（SOD）水平。另外，通过苏木精-伊红染色进行病理学检查。采用免疫组化法检测肝组织中丙二醛（MDA）、超氧化物歧化酶（SOD）含量及核因子（NF）-κB、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β的表达水平。逆转录-定量聚合酶链反应检测细胞色素P450 2 E1（CYP 2 E1）mRNA的表达。结果表明，灵芝提取物可改善肝组织病理学状态，降低血清ALT、AST、TG、TC和MDA水平。同时，乙醇还能降低肝组织中MDA和炎症介质（NF-κB、TNF-α和IL-1β）的水平，并诱导CYP 2 E1 mRNA的过度表达。结果表明，枸杞子提取物对酒精性肝损伤具有一定的保护作用，其机制可能与抗炎、抗氧化作用有关。
Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root of Lindera aggregata (Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury. In the present study, LR extracts were made using various solvents, and then administrated to rats to establish a model of ethanol-induced liver injury. The study aimed to investigate the therapeutic effects and potential mechanism of LR extracts on acute alcoholic liver injury. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycercide (TG), cholesterol (TC), methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) were determined using an automatic biochemistry analyzer. In addition, pathological examination was performed by hematoxylin-eosin staining. The levels of MDA and SOD, and the expression levels of nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver tissue were investigated immunohistochemically. The expression of cytochrome P450 2E1 (CYP2E1) mRNA was quantified by reverse transcription-quantitative polymerase chain reaction. The results indicated that LR extracts improved the histopathological status and decreased the serum levels of ALT, AST, TG, TC and MDA. Furthermore, the levels of MDA and inflammatory mediators (NF-κB, TNF-α and IL-1β) were decreased in liver tissues, and the overexpression of CYP2E1 mRNA induced by ethanol treatment. LR extracts exhibited a protective effect on alcoholic liver injury and the mechanism may be associated with the anti-inflammatory and anti-oxidative action.