Murine Model of Polymicrobial Septic Peritonitis Using Cecal Ligation and Puncture (CLP)
Murine Model of Polymicrobial Septic Peritonitis Using Cecal Ligation and Puncture (CLP)
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DOI：
10.1007/978-1-60761-058-8_23
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Medina, Eva
中科院分区：
文献类型：
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作者：
Medina, Eva
虽然许多动物模型，如内毒素休克和菌血症已被用于研究脓毒症的发病机制，盲肠结扎和穿刺（CLP）是一种腹膜炎模型，具有与人类腹膜炎相似的多微生物感染的临床特征。CLP包括对小鼠的legated盲肠进行手术穿孔，导致盲肠细菌立即持续引流到腹膜腔中。疾病的严重程度取决于用于穿孔的针的直径以及盲肠穿刺的数量。小鼠中脓毒症的CLP模型是研究脓毒症腹膜炎过程的最常用模型，并且可用作临床前模型来测试用于治疗脓毒症的药理学试剂的功效。
Although a number of animal models such as endotoxic shock and bacteremia have been used to study the pathogenesis of sepsis, cecal ligation and puncture (CLP) represents a peritonitis model with clinical features of polymicrobial infection comparable with those of peritonitis in humans. The CLP consists in the surgical perforation of the legated cecum of mice that results in immediate and constant drainage of cecal bacteria into the peritoneal cavity. The severity of the diseases depends on the diameter of the needle used for the perforation as well as on the number of cecal Punctures. The CLP model of sepsis in mice is the most commonly used for studying the process of septic peritonitis and can be used as a preclinical model to test the efficacy of pharmacological agents for the treatment of sepsis.