Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight
Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight
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DOI：
10.1038/ng.3706
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发表时间：
2016-12-01
期刊：
影响因子：
30.8
通讯作者：
Gill, Bikram S.
中科院分区：
文献类型：
--
作者：
Rawat, Nidhi;Pumphrey, Michael O.;Gill, Bikram S.
赤霉病（FHB）由禾谷镰刀菌引起，是小麦和大麦的一种毁灭性病害，会导致谷物减产和霉菌毒素污染，使其不适合人类食用。 FHB 是一个全球性问题，在美国、加拿大、欧洲、亚洲和南美洲都有爆发。仅在美国，1993 年至 2001 年 FHB 造成的直接和次要经济损失总额估计就达 76.7 亿美元(1)。 Fhb1 是 FHB 抗性育种报道最一致的数量性状基因座 (QTL)。在这里，我们报告了从中国小麦品种苏麦3号中基于图位克隆Fhb1。通过突变分析、基因沉默和转基因过表达，我们表明Fhb1上的成孔毒素样（PFT）基因赋予FHB抗性。预计 PFT 编码具有两个凝集素结构域和一个 ETX/MTX2 毒素结构域的嵌合凝集素。我们的发现确定了一种新型持久植物抗性基因，赋予植物对镰刀菌属物种的定量抗病性。
Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of wheat and barley that leads to reduced yield and mycotoxin contamination of grain, making it unfit for human consumption. FHB is a global problem, with outbreaks in the United States, Canada, Europe, Asia and South America. In the United States alone, total direct and secondary economic losses from 1993 to 2001 owing to FHB were estimated at $7.67 billion(1). Fhb1 is the most consistently reported quantitative trait locus (QTL) for FHB resistance breeding. Here we report the map-based cloning of Fhb1 from a Chinese wheat cultivar Sumai 3. By mutation analysis, gene silencing and transgenic overexpression, we show that a pore-forming toxin-like (PFT) gene at Fhb1 confers FHB resistance. PFT is predicted to encode a chimeric lectin with two agglutinin domains and an ETX/MTX2 toxin domain. Our discovery identifies a new type of durable plant resistance gene conferring quantitative disease resistance to plants against Fusarium species.