Redox modulated hydrogelation of a self-assembling short peptide amphiphile
Redox modulated hydrogelation of a self-assembling short peptide amphiphile
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自组装短肽两亲物的氧化还原调节水凝胶
DOI：
10.1007/s11434-012-5487-2
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发表时间：
2012-11-01
影响因子：
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通讯作者：
Lu, Jian R.
中科院分区：
文献类型：
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作者：
Cao ChangHai;Cao MeiWen;Lu, Jian R.
由小肽自组装产生的水凝胶是智能纳米生物材料，因为它们的纳米结构可以很容易地通过环境刺激（例如pH、离子强度和温度）进行调节，从而有利于它们的实际应用。这项工作报告了响应氧化还原环境形成肽水凝胶的实验观察。 Ac-I3K-NH2 是一种短肽两亲物，很容易自组装成长纳米纤维，其凝胶形成发生在浓度约为 10 mmol/L 时。将Cys残基引入亲水区域会产生一种新分子Ac-I(3)CGK-NH2，它能够在自组装纳米纤维之间形成二硫键，从而有利于交联并促进水凝胶的形成。在氧化环境下，Ac-I(3)CGK-NH2 在低得多的浓度（甚至0.5 mmol/L）下形成水凝胶。此外，通过氧化和还原条件和时间的切换，可以轻松调节水凝胶的强度。然而，AFM、TEM 和 CD 测量显示分子和纳米尺寸的形态和结构变化很小，表明二硫键形成没有产生明显的影响。
Hydrogels resulting from the self-assembly of small peptides are smart nanobiomaterials as their nanostructuring can be readily tuned by environmental stimuli such as pH, ionic strength and temperature, thereby favoring their practical applications. This work reports experimental observations of formation of peptide hydrogels in response to the redox environment. Ac-I3K-NH2 is a short peptide amphiphile that readily self-assembles into long nanofibers and its gel formation occurs at concentrations of about 10 mmol/L. Introduction of a Cys residue into the hydrophilic region leads to a new molecule, Ac-I(3)CGK-NH2, that enables the formation of disulfide bonds between self-assembled nanofibers, thus favoring cross-linking and promoting hydrogel formation. Under oxidative environment, Ac-I(3)CGK-NH2 formed hydrogels at much lower concentrations (even at 0.5 mmol/L). Furthermore, the strength of the hydrogels could be easily tuned by switching between oxidative and reductive conditions and time. However, AFM, TEM, and CD measurements revealed little morphological and structural changes at molecular and nano dimensions, showing no apparent influence arising from the disulfide bond formation.