Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.
Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.
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DOI：
10.4142/jvs.2016.17.1.97
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发表时间：
2016-03
影响因子：
1.8
通讯作者：
Kim HY
中科院分区：
文献类型：
--
作者：
Chung HJ;Chung WH;Lee JH;Chung DJ;Yang WJ;Lee AJ;Choi CB;Chang HS;Kim DH;Suh HJ;Lee DH;Hwang SH;Do SH;Kim HY
我们在45只大鼠中使用球囊导管诱导经皮脊髓损伤（SCI），并在损伤部位移植人脐带血来源的间充质干细胞（hUCB-MSCs）。hUCB-MSCs移植组运动功能明显改善。从整个损伤的脊髓提取物的定量ELISA显示脑源性神经营养因子（BDNF），神经生长因子（NGF）和神经营养因子-3（NT-3）的表达增加。我们的研究结果表明，用hUCB-MSCs治疗SCI可以改善运动功能，并表明损伤脊髓中BDNF，NGF和NT-3水平的增加是主要的治疗效果。
We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI with hUCB-MSCs can improve locomotor functions, and suggest that increased levels of BDNF, NGF and NT-3 in the injured spinal cord were the main therapeutic effect.