Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway (Retracted Article)
Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway (Retracted Article)
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DOI：
10.1038/s41419-019-1560-y
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发表时间：
2019-05-10
影响因子：
9
通讯作者：
Wang, Shujing
中科院分区：
文献类型：
--
作者：
Han, Yang;Zhang, Lin;Wang, Shujing
据报道，壳聚糖低聚糖具有抑制多种肿瘤的作用。然而，水溶性海洋植物低聚糖海藻酸寡糖（AOS）具有抗癌作用的报道很少。此外，AOS对前列腺癌的抑制作用及其分子机制尚不清楚。本研究表明，AOS抑制细胞生长，这与α 2,6-唾液化修饰的衰减一致。此外，AOS抑制ST6Gal-1启动子活性，从而影响转录过程。此外，AOS可以激活Hippo/YAP通路，阻断辅激活因子YAP和c-Jun的募集。此外，YAP与转录因子c-Jun相互作用，调节下游靶基因ST6Gal-1的转录活性。与体外实验数据一致，AOS在体内通过Hippo/YAP通路抑制前列腺癌细胞的致瘤性。综上所述，这些数据表明，AOS减缓了前列腺癌的增殖，为传统认知中海带的健康功能提供了依据。
Chitosan oligosaccharides have been reported to inhibit various tumors. However, the water-soluble marine plant oligosaccharide alginate oligosaccharide (AOS) has only rarely been reported to have anti-cancer effects. Moreover, the inhibitory effect of AOS on prostate cancer and the underlying molecular mechanism remain unknown. This study shows that AOS inhibited cell growth, which was consistent with the attenuation of alpha 2,6-sialylation modification. Furthermore, AOS inhibited ST6Gal-1 promoter activity and thus affected transcriptional processes. In addition, AOS could activate the Hippo/YAP pathway and block the recruitment of both the coactivator YAP and c-Jun. Furthermore, YAP interacted with the transcription factor c-Jun and regulated the transcriptional activity of the downstream target ST6Gal-1 gene. Consistent with in vitro data, AOS suppressed the tumorigenicity of prostate cancer cells via the Hippo/YAP pathway in vivo. In summary, these data indicate that AOS slows the proliferation of prostate cancer and provides a basis for the healthy function of kelp in traditional cognition.