Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging.
Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging.
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DOI：
10.1364/ol.37.000362
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发表时间：
2012-02-01
期刊：
影响因子：
3.6
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Xi J;Chen Y;Zhang Y;Murari K;Li MJ;Li X
我们报告了一种全光纤扫描，多模态内窥镜能够同时光学相干层析成像（OCT）和双光子荧光（TPF）成像。这两种成像模式共享相同的微型光纤扫描显微镜，其由双包层光纤组成，该双包层光纤具有在OCT（1310 nm）和双光子激发（1550 nm）波长下以单模操作的芯、压电二维光纤光束扫描器和微型非球面复合透镜，该微型非球面复合透镜适用于同时采集正面OCT和TPF图像。在集成平台中采用光纤波分复用器将低相干OCT光源和飞秒双光子激发激光联合收割机组合到同一光路中。细胞培养物和小鼠离体组织的初步成像结果首次证明了在扫描显微内镜设置中同时进行OCT和TPF实时成像的可行性。
We report an all-fiber-optic scanning, multimodal endomicroscope capable of simultaneous optical coherence to-mography (OCT) and two-photon fluorescence (TPF) imaging. Both imaging modalities share the same miniature fiber-optic scanning endomicroscope, which consists of a double-clad fiber with a core operating in single mode at both the OCT (1310 nm) and two-photon excitation (1550 nm) wavelengths, a piezoelectric two-dimensional fiber-optic beam scanner, and a miniature aspherical compound lens suitable for simultaneous acquisition of en face OCT and TPF images. A fiber-optic wavelength division multiplexer was employed in the integrated platform to combine the low coherence OCT light source and the femtosecond two-photon excitation laser into the same optical path. Preliminary imaging results of cell cultures and mouse tissue ex vivo demonstrate the feasibility of simultaneous real-time OCT and TPF imaging in a scanning endomicroscopy setting for the first time.