Protection in a mouse peritonitis model mediated by iron-regulated outer-membrane protein of Salmonella typhi coupled to its Vi antigen
Protection in a mouse peritonitis model mediated by iron-regulated outer-membrane protein of Salmonella typhi coupled to its Vi antigen
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DOI：
10.1099/jmm.0.05378-0
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发表时间：
2004-07-01
影响因子：
3
通讯作者：
Bhardwaj, SB
中科院分区：
文献类型：
--
作者：
Chibber, S;Bhardwaj, SB
从伤寒沙门氏菌标准菌株 Ty2 中提取并纯化 Vi 多糖和铁调节外膜蛋白 (IROMP)。然后使用碳二亚胺方法通过化学偶联将它们缀合。测试了 Vi-IROMP 缀合物在不同器官中防止伤寒沙门氏菌定植的能力。用 2-5 杯 Vi-IROMP 缀合物免疫的小鼠显示出最大的保护作用，因为观察到脾脏、肝脏和派尔氏集结的细菌定植最少。从经缀合物处理的小鼠获得的腹膜巨噬细胞有效地吞噬细菌。在经缀合物处理的动物中，循环抗体和通过小鼠足垫肿胀阐明的迟发型超敏反应显着较高。这些结果清楚地表明，由同一菌株制备的 IROMP 和伤寒沙门氏菌多糖缀合物具有保护动物免受攻击的潜力。
Vi polysaccharide and iron-regulated outer-membrane proteins (IROMPs) were extracted and purified from the standard strain of Salmonella typhi, Ty2. These were then conjugated by chemical coupling using the carbodimide method. Vi-IROMP conjugate was tested for its ability to protect against colonization by S. typhi in different organs. Mice immunized with 2-5 mug Vi-IROMP conjugate showed the most protection, as the least bacterial colonization of spleen, liver and Peyer's patches was observed. Peritoneal macrophages obtained from conjugate-treated mice phagocytosed bacteria efficiently. Circulating antibodies and the delayed type hypersensitivity response elucidated by mouse foot-pad swelling was significantly higher in conjugate-treated animals. These results clearly demonstrate that an IROMP and polysaccharide conjugate of S. typhi prepared from the same strain has the potential to protect animals against challenge.