A FLUOROMETRIC-DETERMINATION OF N-METHYL-4-PHENYLPYRIDINIUM ION, USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
A FLUOROMETRIC-DETERMINATION OF N-METHYL-4-PHENYLPYRIDINIUM ION, USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/0003-2697(87)90431-3
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发表时间：
1987-05-01
影响因子：
2.9
通讯作者：
NAGATSU, T
中科院分区：
文献类型：
--
作者：
NAOI, M;TAKAHASHI, T;NAGATSU, T
利用荧光和高效液相色谱 (HPLC) 设计了一种简单而灵敏的定量 N-甲基-4-苯基吡啶鎓离子 (MPP+)（一种神经毒素）的测定程序。 MPP+的荧光强度比MPP+的代谢前体N-甲基-1,2,3,6-四氢吡啶（MPTP）强数千倍。该方法的灵敏度足以在不使用 HPLC 的情况下测量小于 10 pmol MPP+ 的量，而使用 HPLC 则测量小于 10 fmol 的量。通过该测定方法检测了人脑突触体线粒体中单胺氧化酶对 MPTP 的氧化。这种荧光高效液相色谱法应该在神经毒素 MPP+ 的研究中具有广泛的应用。
A simple and sensitive assay procedure for the quantitation of N-methyl-4-phenylpyridinium ion (MPP+), a neurotoxin, was devised using its fluorescence and high-performance liquid chromatography (HPLC). The fluorescence intensity of MPP+ was several thousand times more than that of N-methyl-1,2,3,6-tetrahydropyridine (MPTP), a metabolic precursor of MPP+. This method was found to be sensitive enough to measure less than 10 pmol MPP+ without using HPLC and 10 fmol using HPLC. The oxidation of MPTP by monoamine oxidase in human brain synaptosomal mitochondria was examined by this assay method. This fluorometric-HPLC method should have broad application in the study of the neurotoxin MPP+.