Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human
Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human
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DOI：
10.1023/a:1009271706488
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发表时间：
2000-01-01
影响因子：
2.6
通讯作者：
Dutrillaux, B
中科院分区：
文献类型：
--
作者：
Bernardino, J;Lombard, M;Dutrillaux, B
性染色体的DNA甲基化分析使用抗5-甲基胞嘧啶抗体的中期染色体的体细胞从三个物种：人，狐猴和小鼠。还在雄性小鼠中研究了生殖细胞。在雌性细胞（人类和小鼠）中，晚期复制的X染色体总是甲基化较少的染色体。与常染色体相比，成纤维细胞中两条X染色体的甲基化程度总是低于淋巴细胞，小鼠中的差异总是大于人。在人类、小鼠和狐猴的雄性细胞中，独特的X染色体的标记与雌性细胞中早期复制的X染色体的标记非常相似。除了人类Y染色体的异染色质区域高度甲基化外，Y染色体的总体甲基化水平较低。在小鼠睾丸细胞中，根据细胞类型和分化状态观察到多种DNA甲基化模式。最后，在所有条件下保持甲基化的性染色体的唯一结构对应于它们的假常染色体区域。
DNA methylation of sex chromosomes was analysed using anti-5-methylcytosine antibodies on metaphase chromosomes of somatic cells from three species: human, lemur and mouse. Germ cells were also studied in male mouse. In female cells (human and mouse), the late replicating X was always the less methylated chromosome. Compared with autosomes, the methylation of both X chromosomes was always lower in fibroblasts than in lymphocytes and the difference was always greater in mouse than in human. In human, mouse and lemur male cells, the labelling of the unique X chromosome was quite similar to that of the early replicating X from female cells. Except for the heterochromatic region of the human Y chromosome, strongly methylated, the overall methylation of the Y chromosome was low. In mouse testicular cells, a variety of DNA methylation patterns was observed according to the cell type and the state of differentiation. Finally, the only structures of sex chromosomes which remain methylated in all conditions correspond to their pseudoautosomal regions.