Parentage Analysis in Japanese Peaches using SSR Markers.
Parentage Analysis in Japanese Peaches using SSR Markers.
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使用 SSR 标记对日本桃子进行亲子关系分析。
DOI：
10.1270/jsbbs.53.35
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Tateki Hayashi
中科院分区：
文献类型：
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作者：
Toshiya Yamamoto;Kouhei Mochida;T. Imai;T. Haji;H. Yaegaki;M. Yamaguchi;N. Matsuta;I. Ogiwara;Tateki Hayashi
利用17个SSR标记对16个桃品种（包括9个控制杂交品种、2个芽变品种和5个随机实生品种）的亲缘关系进行了分析。9个杂交品种的SSR等位基因均能从亲本传递到后代品种中，在所有位点上均无差异，证实了杂交品种的亲子关系。‘晓’和‘结生’的SSR基因型完全相同，表明‘结生’来源于芽变突变。另一个芽变品种‘北川白广’在12个SSR位点上表现出与原始品种‘白广’不同的SSR基因型，表明‘北川白广’不是‘白广’的芽变突变体。四个品种，可能起源于机会幼苗，即，‘阿部白藤’、‘川中岛白藤’、‘光阳白藤’和‘清水白藤’在每个SSR位点上与它们的推定亲本‘白藤’共有一个等位基因。SSR分析表明，这4个品种不是芽变突变体，而是‘白龙’的后代。SSR标记可有效地用于日本栽培桃的亲缘关系分析。
Parentage of 16 peach cultivars, including 9 cultivars bred by controlled hybridization, 2 from bud sport mutations and 5 from chance seedlings, was analyzed using 17 SSR markers. The parent-offspring relationships of 9 crossbreeding cultivars were confirmed because SSR alleles were transmitted to offspring cultivars from their parents without discrepancy at all the used loci. ‘Akatsuki’ and ‘Gyousei’ showed identical SSR genotypes, which suggested that ‘Gyousei’ was derived from a bud sport mutation. In contrast, another bud sport cultivar, ‘Hikawa Hakuhou’, showed different SSR genotypes from those of the original cultivar ‘Hakuhou’ at 12 SSR loci, indicating that ‘Hikawa Hakuhou’ was not a bud sport mutant of ‘Hakuhou’. Four cultivars that presumably originated from chance seedlings, i.e., ‘Abe Hakutou’, ‘Kawanakajima Hakutou’, ‘Kouyou Hakutou’ and ‘Shimizu Hakutou’, shared one allele with their putative parent ‘Hakutou’ at each SSR locus. SSR analysis suggested that these 4 cultivars were not bud sport mutants, but offsprings of ‘Hakutou’. It was concluded that SSR markers could be effectively utilized for parentage analysis in Japanese cultivated peaches.