Medical-grade collagen peptide in injectables provides antioxidant protection
Medical-grade collagen peptide in injectables provides antioxidant protection
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DOI：
10.3109/10837450.2013.860547
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发表时间：
2015-02
影响因子：
3.4
通讯作者：
Kyo Kobayashi;Y. Maehata;Yasue Okada;M. Kusubata;S. Hattori;Keisuke Tanaka;C. Miyamoto;Fumihiko Yoshino-Fum
中科院分区：
文献类型：
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作者：
Kyo Kobayashi;Y. Maehata;Yasue Okada;M. Kusubata;S. Hattori;Keisuke Tanaka;C. Miyamoto;Fumihiko Yoshino-Fum
摘要医用级胶原蛋白多肽被用作药物制剂的添加剂，但该化合物是否具有体外抗氧化作用尚不清楚。在本研究中，我们利用电子自旋共振和自旋捕捉法研究了医用级胶原蛋白多肽对氧自由基、超氧阴离子自由基和单线态氧的抗氧化作用。我们证实，医用级胶原蛋白多肽直接抑制Fenton反应或过氧化氢紫外线照射产生的羟基自由基，以及单线态氧。此外，还观察到了医用级胶原蛋白多肽对12-22肽组分中单线态氧的抗氧化作用。抗氧化性氨基酸总量(Gly、Hyp、Glu、Ala、Cys、Met和His)占总氨基酸的一半以上。这些结果表明，医用级胶原蛋白多肽的抗氧化性能是由于含有抗氧化氨基酸的化合物所致。医用级胶原蛋白多肽用于制药，特别是注射剂中，可以提供有用的抗氧化性，以保护活性成分免受氧化。
Abstract Medical-grade collagen peptide is used as an additive agent in pharmaceutical formulations; however, it is unknown as to whether the compound exerts antioxidant effects in vitro. In this study, we evaluated the antioxidant effects of medical-grade collagen peptide on reactive oxygen species such as hydroxyl radical, superoxide anion radical and singlet oxygen using electron spin resonance and spin trapping. We confirmed that medical-grade collagen peptide directly inhibited hydroxyl radical generated by the Fenton reaction or by ultraviolet irradiation of hydrogen peroxide, and singlet oxygen. In addition, an antioxidant effect of medical-grade collagen peptide on singlet oxygen was observed in peptide fractions 12–22. The total amount of antioxidant amino acids (Gly, Hyp, Glu, Ala, Cys, Met and His) constituted more than half of the total amino acids in these fractions. These results suggest that the observed antioxidant properties of medical-grade collagen peptide are due to the compound containing antioxidant amino acids. Medical-grade collagen peptide, which is used in pharmaceuticals, and especially in injectables, could provide useful antioxidant properties to protect the active ingredient from oxidation.