Establishment and characterization of rat cell lines transformed by restriction endonuclease fragments of adenovirus 12 DNA.
Establishment and characterization of rat cell lines transformed by restriction endonuclease fragments of adenovirus 12 DNA.
复制标题
腺病毒 12 DNA 限制性内切酶片段转化的大鼠细胞系的建立和表征。
DOI：
10.1016/0042-6822(77)90019-8
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发表时间：
1977
期刊：
影响因子：
3.7
通讯作者：
K. Fujinaga
中科院分区：
文献类型：
--
作者：
K. Shiroki;H. Handa;H. Shimojo;S. Yano;S. Ojima;K. Fujinaga
用腺病毒12 DNA的HindIII-G片段转化大鼠细胞（3 Y1），并在软琼脂培养基上克隆，建立了转化细胞系（GY 1、GY 2和GY 3细胞）。腺病毒12 DNA的部分HindIII-G片段是存在于GY 1细胞中的唯一病毒DNA序列。检测了CY 1（腺病毒12 DNA的EcoRI-C片段转化的大鼠细胞系）、GY 1和WY 3细胞（腺病毒12的全病毒DNA转化的大鼠细胞系）的特性。与未转化的3 Y1细胞相比，每个转化细胞系的生长是无限的。补体结合和免疫荧光显示CY 1和GY 1细胞含有T抗原，而WY 3细胞既含有T抗原又含有DNA结合蛋白。CY 1、GY 1和WY 3细胞移植后在大鼠中诱发肿瘤。在携带CY 1或GY 1细胞肿瘤的大鼠的血清中，仅检测到T抗原的抗体，而携带WY 3细胞肿瘤的大鼠的血清中存在T抗原和病毒诱导的DNA结合蛋白的抗体。这些结果表明，转化基因位于腺病毒12 DNA的左端（7.2%），并表明T抗原是由该基因组区域编码的基因产物之一。
Rat cells (3Y1) were transformed by theHindIII-G fragment of adenovirus 12 DNA and cloned in solft agar cultures, and transformed cell lines (GY1, GY2, and GY3 cells) were established. Part of theHindIII-G fragment of adenovirus 12 DNA was the only viral DNA sequence present in GY1 cells. The characteristics of CY1 (a rat cell line transformed by theEcoRI-C fragment of adenovirus 12 DNA), GY1, and WY3 cells (a rat cell line transformed by the whole viral DNA of adenovirus 12) were examined. The growth of each transformed cell line was unlimited as compared to that of untransformed 3Y1 cells. Complement fixation and immunofluorescence suggested that CY1 and GY1 cells contained T antigen, while WY3 cells contained both T antigen and DNA-binding protein. CY1, GY1, and WY3 cells induced tumors in rats after transplantation. In the serum of rats bearing CY1 or GY1 cell tumors only antibody to T antigen was detectable, while antibodies to both T antigen and virus-induced DNA-binding protein were present in the serum of rats bearing WY3 cell tumors. These results show that the transforming gene is located on the left end (7.2%) of adenovirus 12 DNA and suggest that T antigen is among the gene products coded for by this region of the genome.