A CHLOROPLAST DNA INVERSION MARKS AN ANCIENT EVOLUTIONARY SPLIT IN THE SUNFLOWER FAMILY (ASTERACEAE)
A CHLOROPLAST DNA INVERSION MARKS AN ANCIENT EVOLUTIONARY SPLIT IN THE SUNFLOWER FAMILY (ASTERACEAE)
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DOI：
10.1073/pnas.84.16.5818
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
PALMER, JD
中科院分区：
文献类型：
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作者：
JANSEN, RK;PALMER, JD
我们确定了叶绿体DNA倒置分布在80个物种代表16个部落的菊科和10个推定相关的科。利用克隆的莴苣和矮牛叶绿体DNA限制性片段进行过滤杂交，发现这一22千碱基对反转在57个属中共有，代表了菊科的所有部落，但在菊科的Barnadesiinae亚部落以及与菊科相关的所有科中都没有。这种倒置定义了这个家族中一个古老的进化分裂，并表明Barnadesiinae代表了Asteraceae中最原始的谱系。这些结果也表明multisiae部落不是单系的，因为它的四个亚部落的任何共同祖先也被该家族的其他部落共享。这是对细胞器DNA重排的系统分布的最广泛的调查，并证明了这种突变在更高的分类水平上解决系统发育关系的潜力。
We determined the distribution of a chloroplast DNA inversion among 80 species representing 16 tribes of the Asteraceae and 10 putatively related families. Filter hybridizations using cloned chloroplast DNA restriction fragments of lettuce and petunia revealed that this 22-kilobase-pair inversion is shared by 57 genera, representing all tribes of the Asteraceae, but is absent from the subtribe Barnadesiinae of the tribe Mutisieae, as well as from all families allied to the Asteraceae. The inversion thus defines an ancient evolutionary split within the family and suggests that the Barnadesiinae represents the most primitive lineage in the Asteraceae. These results also indicate that the tribe Mutisieae is not monophyletic, since any common ancestor to its four subtribes is also shared by other tribes in the family. This is the most extensive survey of the systematic distribution of an organelle DNA rearrangement and demonstrates the potential of such mutations for resolving phylogenetic relationships at higher taxonomic levels.