Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115.
Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115.
复制标题
霍尔金人的顺序绑扎和囊泡螺旋蛋白P115的snrepin组装催化。
DOI：
10.1083/jcb.200112127
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发表时间：
2002-04-01
影响因子：
7.8
通讯作者：
Warren, Graham
中科院分区：
文献类型：
--
作者：
Shorter, James;Beard, Matthew B;Seemann, Joachim;Dirac-Svejstrup, A Barbara;Warren, Graham
p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28–syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).
