Haemophilus ducreyi infection causes basal keratinocyte cytotoxicity and elicits a unique cytokine induction pattern in an In vitro human skin model.
Haemophilus ducreyi infection causes basal keratinocyte cytotoxicity and elicits a unique cytokine induction pattern in an In vitro human skin model.
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杜克雷嗜血杆菌感染会引起基础角质形成细胞的细胞毒性，并在体外人类皮肤模型中引发独特的细胞因子诱导模式。
DOI：
10.1128/iai.66.6.2914-2921.1998
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发表时间：
1998
影响因子：
3.1
通讯作者：
Kawula,TH
中科院分区：
文献类型：
--
作者：
Hobbs,MM;Paul,TR;Wyrick,PB;Kawula,TH
杜氏嗜血杆菌是性传播性生殖器溃疡软下疳的病原。主要是皮肤病原体，H。ducreyii存在于软下疳溃疡中，其特征是表皮内病变中大量中性粒细胞积聚，并伴有真皮单核细胞浸润。我们用包皮成纤维细胞和角质形成细胞组成的体外人皮肤模型来检测宿主皮肤细胞与h的相互作用。ducreyi35000。细菌在人造皮肤中复制并存活了至少14天。我们observedH。透射电镜观察基底上角质形成细胞内的ducreyi。虽然在基底角化细胞区未见细菌，但这些细胞在感染的共培养中被破坏。ducrei感染刺激皮肤细胞分泌白细胞介素-6 （IL-6）和IL-8。相反，肿瘤坏死因子α和IL-1α水平未升高。IL-8的产生响应于h。ducrei感染可能参与募集多形核白细胞和其他炎症细胞，从而导致组织坏死和溃疡形成的软下疳特征。
Haemophilus ducreyiis the etiologic agent of the sexually transmitted genital ulcer disease chancroid. Predominantly a cutaneous pathogen,H. ducreyiis present in chancroid ulcers that are characterized by extensive neutrophil accumulation in intraepidermal lesions accompanied by a mononuclear infiltrate in the dermis. We used an in vitro human skin model composed of foreskin fibroblasts and keratinocytes to examine host skin cell interactions withH. ducreyi35000. Bacteria replicated and persisted in artificial skin for at least 14 days. We observedH. ducreyiinside suprabasal keratinocytes using transmission electron microscopy. Although no bacteria were seen in the basal keratinocyte region, these cells were disrupted in infected cocultures.H. ducreyiinfection stimulated increased secretion of interleukin-6 (IL-6) and IL-8 by skin cells. Conversely, tumor necrosis factor alpha and IL-1α levels were not elevated. IL-8 produced in response toH. ducreyiinfection may be involved in recruiting polymorphonuclear leukocytes and other inflammatory cells, thereby contributing to the tissue necrosis and ulcer formation characteristic of chancroid.