Mechanism of oleic acid-induced inhibition on gastric acid secretion in rats.
Mechanism of oleic acid-induced inhibition on gastric acid secretion in rats.
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油酸抑制大鼠胃酸分泌的机制。
DOI：
10.1152/ajpgi.1991.260.4.g564
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Chey,WY
中科院分区：
文献类型：
--
作者：
Rhee,JC;Chang,TM;Lee,KY;Jo,YH;Chey,WY
我们研究了存在的肠抑胃素诱导的大鼠十二指肠给药的油酸。通过静脉输注0.3 μ g·kg ~（-1）·h ~（-1）五肽胃泌素刺激麻醉大鼠的腔灌注胃分泌酸。十二指肠内注射3 mmol油酸对10只大鼠五肽胃泌素刺激的酸分泌有显著抑制作用（94%）（P <0.01）。在血浆中的几种肽，包括促胰液素，神经降压素，生长抑素，和肽YY，只有促胰液素被发现显着增加（P <0.001）。猪促胰液素（5.6 pmol.kg-1. h-1）静脉给药以模拟油酸输注产生的促胰液素血浆水平，引起了类似程度的酸排出抑制（93%）。油酸对酸分泌的抑制作用可被兔抗胰泌素血清完全逆转，但不能被正常兔血清逆转。这些观察结果强烈表明抑制作用是通过循环分泌素介导的。吲哚美辛可完全阻断油酸或胰泌素的抑制作用。静脉注射48 μ g·kg-1·h-1前列腺素E2可完全逆转阻断作用。我们的结论是内源性促胰液素是一个主要的肠抑胃素释放的油酸在麻醉大鼠和促胰液素的抑制作用需要内源性胰高血糖素。
We investigated the existence of an enterogastrone in rats induced by duodenal administration of oleic acid. Acid secretion by the luminally perfused stomach was stimulated in anesthetized rats by intravenous infusion of 0.3 micrograms.kg-1.h-1 pentagastrin. Intraduodenal administration of 3 mmol of oleic acid produced a profound inhibition (94%) of pentagastrin-stimulated acid output in 10 rats (P less than 0.01). Of several peptides in plasma including secretin, neurotensin, somatostatin, and peptide YY, only secretin was found to increase significantly (P less than 0.001). A similar degree of inhibition of acid output (93%) was caused by porcine secretin, 5.6 pmol.kg-1.h-1, given intravenously to mimic the plasma level of secretin produced by oleic acid infusion. The inhibitory effect of oleic acid on the acid secretion was completely reversed by intravenous injection of a rabbit antisecretin serum but not by a normal rabbit serum. These observations strongly suggest that the inhibition was mediated via circulating secretin. The inhibition produced by either oleic acid or secretin was completely blocked by indomethacin. The blocking action was completely reversed by intravenous administration of 48 micrograms.kg-1.h-1 prostaglandin E2. We conclude that endogenous secretin is a major enterogastrone released by oleic acid in anesthetized rats and that the inhibitory action of secretin requires endogenous prostaglandins.