Chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of rat tibial articular cartilage
Chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of rat tibial articular cartilage
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大鼠胫骨关节软骨发育过程中软骨细胞合成I型胶原并在基质中积累蛋白质
DOI：
10.1007/bf00187135
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
M. Kagayama
中科院分区：
文献类型：
--
作者：
Y. Sasano;M. Furusawa;H. Ohtani;I. Mizoguchi;I. Takahashi;M. Kagayama
本研究旨在探讨在生理条件下关节软骨中的软骨细胞是否在体内表达I型胶原。在发育过程中的大鼠膝关节的胫骨关节软骨中检测基因的表达和I型胶原的表型。将出生后1、5和11周的Wistar大鼠膝关节固定在4%多聚甲醛（含或不含0.5%戊二醛）中，并在10%EDTA中脱钙。石蜡包埋后连续切片，相邻切片行I型胶原免疫组化和原位杂交。第1周大鼠胫骨骨骺由软骨组成。随着软骨内骨化的进行，关节软骨的形成在第5周进行，并在第11周完成。抗I型胶原抗体染色的骨骺在第1周的浅表区域，但免疫反应扩大到更深的区域的关节软骨的发展在第5周和第11周。在第1周胫骨骨骺的一些软骨细胞中观察到前α 1（I）胶原的杂交信号。在第5周，在软骨细胞中识别出最强的信号，并且在第11周，信号似乎较弱。本研究表明，在关节软骨发育过程中，软骨细胞合成I型胶原并在基质中积累蛋白质。
The present study was designed to investigate whether or not chondrocytes in articular cartilage express type I collagen in vivo under physiological conditions. Expressions of the gene and the phenotype of type I collagen were examined in rat tibial articular cartilage in the knee joint during development. Knee joints of Wistar rats at 1, 5, and 11 weeks postnatal were fixed in 4% paraformaldehyde with or without 0.5% glutaraldehyde and decalcified in 10% EDTA. After the specimens were embedded in paraffin and serial sections made, adjacent sections were processed for immunohistochemistry and in situ hybridization for type I collagen. The epiphysis of the tibia was composed of cartilage in week-1 rats. Formation of articular cartilage was in progress in week 5 as endochondral ossification proceeded and was completed in week 11. Anti-type I collagen antibody stained only the superficial area of the epiphysis in week 1, but the immunoreactivity was expanded into the deeper region of the articular cartilage with development in weeks 5 and 11. Hybridization signals for pro-alpha 1 (I) collagen were seen in some of chondrocytes in the epiphysis of the week-1 tibia. The most intense signals were identified in chondrocytes in week 5 and the signals appeared weaker in week 11. The present study demonstrated that chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of the articular cartilage.
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC