Long-term evolution of antigen repertoires among carried meningococci
Long-term evolution of antigen repertoires among carried meningococci
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DOI：
10.1098/rspb.2009.2033
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发表时间：
2010-06-07
影响因子：
4.7
通讯作者：
Jolley, Keith A.
中科院分区：
文献类型：
--
作者：
Buckee, Caroline O.;Gupta, Sunetra;Jolley, Keith A.
对细菌病原体种群的大多数研究都是基于在短时间内从患病个体或其接触者身上收集的分离物。然而，对于偶尔引起疾病的共生生物，如脑膜炎奈瑟菌，有必要对长期无症状携带的分离株进行分析，以阐明其进化和种群结构。在这里，我们使用数学模型来分析近30年来在捷克共和国收集的携带脑膜炎球菌分离株中三种疫苗候选抗原的结构和动态。数据表明，尽管有证据表明重组率很高，但抗原等位基因的稳定组合在这段时间内得到了维持，这与理论模型一致，即在存在重组的情况下，强免疫选择可以维持抗原决定基因的非重叠组合。我们将这种抗原结构与相同分离株中观察到的重叠但相对稳定的管家基因组合进行了对比，并使用一种新的网络方法来可视化这些关系。
Most studies of bacterial pathogen populations have been based on isolates collected from individuals with disease, or their contacts, over short time periods. For commensal organisms that occasionally cause disease, such as Neisseria meningitidis, however, the analysis of isolates from long-term asymptomatic carriage is necessary to elucidate their evolution and population structure. Here, we use mathematical models to analyse the structuring and dynamics of three vaccine-candidate antigens among carried meningococcal isolates collected over nearly 30 years in the Czech Republic. The data indicate that stable combinations of antigenic alleles were maintained over this time period despite evidence for high rates of recombination, consistent with theoretical models in which strong immune selection can maintain non-overlapping combinations of antigenic determinants in the presence of recombination. We contrast this antigenic structure with the overlapping but relatively stable combinations of the housekeeping genes observed among the same isolates, and use a novel network approach to visualize these relationships.