Localized Induction of the ntcA Regulatory Gene in Developing Heterocysts of Anabaena sp. Strain PCC 7120
Localized Induction of the ntcA Regulatory Gene in Developing Heterocysts of Anabaena sp. Strain PCC 7120
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ntcA 调节基因在鱼腥藻异囊发育中的局部诱导。
DOI：
10.1128/jb.00509-06
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发表时间：
2006
影响因子：
3.2
通讯作者：
A. Herrero
中科院分区：
文献类型：
--
作者：
Elvira Olmedo;A. Muro;E. Flores;A. Herrero
摘要在蓝藻中，NTCA基因编码一个N控制的转录调控因子。在固氮、形成异形胞的蓝藻鱼腥藻中。PCC7120株NTCA基因是由一个复杂的启动子区域转录而来的自我调节基因，该区域包括一个构成启动子(P2)和N阶梯诱导的启动子(P1和P3)。利用NTCA-GFP翻译融合的方法研究了NTCA的表达，该融合既存在于天然的NTCA基因座中，也存在于异源基因组中。NTCA-GFP的诱导发生在所有细胞的N级下降后，但在发育中的异形胞中诱导水平特别高。局部诱导可由P3和P1独立驱动。
ABSTRACT The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N2-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P2) and promoters that are induced upon N step-down (P1 and P3). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P3 and P1.
影响因子：
10.5
作者：
BUIKEMA, WJ;HASELKORN, R
通讯作者：
HASELKORN, R