NCL-SG3: a human eccrine sweat gland cell line that retains the capacity for transepithelial ion transport.
NCL-SG3: a human eccrine sweat gland cell line that retains the capacity for transepithelial ion transport.
复制标题
NCL-SG3：人类小汗腺细胞系，保留跨上皮离子转运的能力。
作者：
C. M. Lee;J. Dessi
用猴病毒40(SV40)感染人汗腺原代培养物，建立了离子传输型人上皮细胞系NCL-SG3。该品系已经传代38次(超过100个种群加倍)，具有非整倍体核型，但没有经历任何‘危机’。这些细胞保留了上皮细胞的形态和细胞角蛋白的表达，这是上皮细胞的中间丝特征。大约85%的人在体内至少有微弱的波形蛋白共表达，波形蛋白是一种与间充质细胞和其他一些非上皮细胞类型相关的中间丝。此外，SV40大T抗原也存在，以核定位为主。耐电池片形成在透析管和纤维素酯可渗透支撑物上。β-肾上腺素能激动剂异丙肾上腺素(10(-6)M)和赖氨酸激动素(10(-7)M)可刺激产生离子转运，而胆碱能激动剂卡巴胆碱(10(-6)M)则不能。
An ion-transporting human epithelial cell line, NCL-SG3, has been established by simian virus 40 (SV40) infection of primary cultures from eccrine sweat glands. The line has been passaged 38 times (over 100 population doublings), has an aneuploid karyotype but has not undergone any 'crisis'. The cells have retained epithelial morphology and expression of cytokeratin, the intermediate filament characteristic of epithelial cells. Approximately 85% of the population shows at least weak co-expression of vimentin, an intermediate filament associated with mesenchymal and some other non-epithelial cell types in vivo. In addition, SV40 large T-antigen is present, in a predominantly nuclear localization. Electrically resistant cell sheets are formed on dialysis tubing and cellulose-ester permeable supports. Electrogenic ion transport can be stimulated by the beta-adrenergic agonist isoproterenol (10(-6) M) and by lysylbradykinin (10(-7) M) but not by the cholinergic agonist carbachol at 10(-6) M).
SV40 对人角质形成细胞的转化和永生化。
DOI：
10.1111/1523-1747.ep12540905
发表时间：
1983
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Steinberg,ML;Defendi,V
通讯作者：
Defendi,V
SV40 大肿瘤抗原的细胞内转运：消除向细胞核迁移的突变不会阻止与细胞表面的结合。
影响因子：
3.7
作者：
Lanford,RE;Butel,JS
通讯作者：
Butel,JS
激肽对猪肾乳头集合管细胞原代培养物中生电离子转运的影响。
DOI：
10.1152/ajprenal.1985.249.3.f439
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
--
作者：
Cuthbert,AW;George,AM;MacVinish,L
通讯作者：
MacVinish,L