Synchronized spikes of thalamocortical axonal terminals and cortical neurons are detectable outside the pig brain with MEG
Synchronized spikes of thalamocortical axonal terminals and cortical neurons are detectable outside the pig brain with MEG
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DOI：
10.1152/jn.00332.2001
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发表时间：
2002-01-01
影响因子：
2.5
通讯作者：
Okada, YC
中科院分区：
文献类型：
--
作者：
Ikeda, H;Leyba, L;Okada, YC
我们表明，它是可行的，以监测同步群体尖峰的丘脑皮层轴突末梢和皮层神经元的大脑外，使用高分辨率脑磁图（MEG）。电刺激猪的口鼻部引起了体细胞诱发的磁场（SEFs）以上的初级躯体感觉皮层（SI）的仔猪。SEF包含600 Hz左右的高频振荡（HFO），在许多方面与MEG和脑电图（EEG）无创测量的HFO相似。这些HFO高度相关的同时测量皮层内体细胞诱发电位（SEP）在口鼻部投影区SI。在SEFs和SEPs中的HFO均由对皮质注射的犬尿烯酸（Kyna，20 mM）不敏感的初始组分（一种非特异性多巴胺能受体拮抗剂）和随后的Kyna敏感组分组成。前者定位于皮质层IV，这表明这是由于特定的丘脑皮质轴突终端产生的尖峰，而后者最初定位于IV层，随后在浅层和深层。这些结果表明，它可能是可能的研究性质的丘脑皮层和皮层锋电位活动在人类与MEG。
We show that it is feasible to monitor the synchronized population spikes of the thalamocortical axonal terminals and cortical neurons outside the brain using high-resolution magnetoencephalography (MEG). Electrical stimulation of the snout elicited somatic-evoked magnetic fields (SEFs) above the primary somatosensory cortex (SI) of the piglet. The SEFs contained high-frequency oscillations (HFOs) around 600 Hz similar in many respects to the noninvasively measured HFOs from humans with MEG and electroencephalography (EEG). These HFOs were highly correlated with those in simultaneously measured intracortical somatic-evoked potentials (SEPs) in the snout projection area in SI. Both HFOs in SEFs and SEPs consisted of an initial component insensitive to cortically injected kynurenic acid (Kyna, 20 mM), a nonspecific antagonist of glutamatergic receptors, and a subsequent Kyna-sensitive component. The former was localized in cortical layer IV, indicating that it was due to spikes produced by the specific thalamocortical axonal terminals, whereas the latter was initially localized in layer IV and subsequently in the superficial and deeper layers. These results suggest that it may be possible to study properties of the thalamocortical and cortical spike activities in humans with MEG.