Development of pepper SSR markers from sequence databases
Development of pepper SSR markers from sequence databases
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DOI：
10.1023/a:1004059722512
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发表时间：
2001-01-01
期刊：
影响因子：
1.9
通讯作者：
Guo, JZ
中科院分区：
文献类型：
--
作者：
Huang, SW;Zhang, BX;Guo, JZ
简单序列重复(SSR)标记在植物育种中具有潜在的应用价值。这项工作的目的是在EMBL和Genbank数据库中搜索辣椒属含有SSR的序列，评估不同基序的频率，并检查一组选定的标记在一组基因型中的多态性，其中包括10种辣椒。番茄和马铃薯各1个。在辣椒序列中发现了58个不同基序的微卫星。选择其中的12个子集，利用SSR侧翼的聚合酶链式反应引物进行多态调查。其中5对引物可检测到辣椒品系间的多态性。用马铃薯的3对引物和番茄的1对引物对预测大小的PCR产物进行了扩增。研究还表明，较短的微卫星可能是辣椒属中有价值的标记。
Simple sequence repeat (SSR) markers are potentially valuable tools for plant breeding. The objectives of the work reported here were to search the EMBL and Genbank databases for the presence of SSR-containing sequences from the genus Capsicum, to assess the frequency of different motifs, and to examine the polymorphism of selected markers in a panel of genotypes, including 10 Capsicum spp. and 1 tomato and 1 potato genotype. Fifty-eight microsatellites with different motifs were found in Capsicum sequences. A subset of twelve of these were selected for the polymorphism survey using PCR primers flanking the SSR. Polymorphisms between Capsicum lines can be detected with 5 of these primer pairs. PCR products of the predicted size were also amplified with three primer pairs in potato and one primer pair in tomato. The study also showed that shorter microsatellites could be valuable markers in Capsicum.