MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN IGE-PRODUCING B-CELLS - A POTENTIAL THERAPEUTIC FOR IGE-MEDIATED ALLERGIC DISEASES
MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN IGE-PRODUCING B-CELLS - A POTENTIAL THERAPEUTIC FOR IGE-MEDIATED ALLERGIC DISEASES
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DOI：
10.1038/nbt0290-122
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发表时间：
1990-02-01
期刊：
影响因子：
--
通讯作者：
HAMILTON, RG
中科院分区：
文献类型：
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作者：
CHANG, TW;DAVIS, FM;HAMILTON, RG
对淋巴细胞表面抗原具有特异性的单克隆抗体（MAbs）被用于体内靶向和清除肿瘤或正常淋巴细胞。在这里，我们报告的证据，存在的抗原表位的IgE，可在IgE分泌B细胞，但不对其他细胞轴承IgE。在42个鼠单克隆抗体特异性的人IgE，两个显示通过荧光流式细胞术分析结合IgE分泌细胞系，但不结合高亲和力IgE的IgE。嗜碱性粒细胞上的Fc受体（Fc∈RI）或其他细胞类型上的低亲和力IgE受体（Fc∈RII）。即使在非常宽松的条件下，它们也不能诱导各种供体的嗜碱性粒细胞释放组胺。这些抗体可用于靶向患有IgE介导的变态反应的患者中的分泌IgE的B细胞。
Monoclonal antibodies (MAbs) specific for surface antigens of lymphocytes are being used to target and deplete tumorous or normal lymphocytesin vivo. Here, we report evidence for the existence of antigenic epitopes on IgE that are accessible on IgE-secreting B cells but not on other cells bearing IgE. Among 42 murine MAbs specific for human IgE, two were shown by fluorescence flow cytometric analyses to bind to IgE-secreting cell lines but not to IgE bound to high-affinity IgE. Fc receptors (Fc∈RI) on basophils or low-affinity IgE receptors (Fc∈RII) on other cell types. Neither could they induce histamine release from basophils of various donors even under very permissive conditions. These antibodies may be useful for targeting IgE-secreting B cells in patients suffering from IgE-mediated allergies.