Evidence for autocrine production of IGF-1 in human thyroid adenomas
Evidence for autocrine production of IGF-1 in human thyroid adenomas
复制标题
人甲状腺腺瘤自分泌产生 IGF-1 的证据
DOI：
10.1016/0303-7207(89)90199-8
复制
发表时间：
1989
影响因子：
4.1
通讯作者：
D. Wynford‐Thomas
中科院分区：
文献类型：
--
作者：
D.Wynne Williams;E. Williams;D. Wynford‐Thomas
我们最近发现，大部分良性人类甲状腺肿瘤（滤泡性腺瘤）的上皮细胞已经摆脱了正常细胞对外源胰岛素样生长因子 1 (IGF-1) 的需求，这表明 IGF-1 诱导的生长信号要么自分泌产生，要么在细胞内产生。我们在这里表明，来自这些腺瘤细胞的条件培养基可以赋予正常甲状腺上皮细胞IGF-1独立性，并且这种活性可以通过抗IGF-1抗体的免疫吸附消除。此外，肿瘤细胞而非正常细胞含有免疫反应性IGF-1。我们的数据提供了良性上皮肿瘤中 IGF-1（或相关因子）自分泌产生的第一个证据，并表明这可能是甲状腺肿瘤发生的关键早期步骤。
We have recently shown that epithelial cells from a high proportion of benign human thyroid tumours (follicular adenomas) have escaped from the requirement of the normal cell for an exogenous source of insulin-like growth factor 1 (IGF-1), suggesting either autocrine production or intracellular generation of an IGF-1-induced growth signal. We show here that conditioned medium from these adenoma cells can confer IGF-1 independence on normal thyroid epithelial cells and that this activity is abolished by immunoadsorption with an anti-IGF-1 antibody. In addition, tumour but not normal cells contain immunoreactive IGF-1. Our data provide the first evidence for autocrine production of IGF-1 (or a related factor) in abenignepithelial tumour and suggest that this may be a key early step in thyroid tumorigenesis.