Diversity and abundance of anammox bacterial community in the deep-ocean surface sediment from equatorial Pacific
Diversity and abundance of anammox bacterial community in the deep-ocean surface sediment from equatorial Pacific
复制标题
赤道太平洋深海表层沉积物中厌氧氨氧化细菌群落的多样性和丰度
DOI：
10.1007/s00253-010-2925-4
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发表时间：
2011-02-01
影响因子：
5
通讯作者：
Zheng, Tian-Ling
中科院分区：
文献类型：
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作者：
Hong, Yi-Guo;Yin, Bo;Zheng, Tian-Ling
通过16S rRNA和联氨氧化还原酶(HZO)基因的系统发育分析和PCoA统计分析，研究了赤道太平洋表层沉积物中厌氧氨氧化(Anammox)细菌的群落结构和多样性。结果表明，P2(西太平洋暖池中心外)和P3(赤道东太平洋)的16S rRNA和hzos序列都属于假丝酵母菌“Scaldua”，这是前人研究证实的低温海洋环境中的优势厌氧氨氧化细菌。然而，在位于西太平洋暖池中心的P1位，大部分16S rRNA基因序列形成了一个独立的簇。同时，来自P1沉积物的第三系层序也聚为一个单独的簇。PCoA分析表明，P1的Anammox群落结构与P2、P3和其他海洋环境中基于16S rRNA和hzgenes的群落结构有显著的地理分布差异。赤道太平洋表层沉积物中厌氧氨氧化细菌的丰度范围为3.98 × 103~1.17 × 104拷贝g−1干沉积物。这些结果表明，西太平洋暖池表层沉积物中存在一种特殊的厌氧氨氧化细菌菌型，这种菌型适应于特定的栖息地，并可能参与了栖息地固定清单的氮素流失过程。
The community structure and diversity of anaerobic ammonium oxidation (anammox) bacteria in the surface sediments of equatorial Pacific were investigated by phylogenic analysis of 16S rRNA and hydrazine oxidoreductase (hzo) genes and PCoA (principal coordinates analysis) statistical analysis. Results indicated that 16S rRNA andhzosequences in the P2 (off the center of western Pacific warm pool) and P3 (in the eastern equatorial Pacific) sites all belong to theCandidatus“Scalindua”, the dominate anammox bacteria in the low-temperature marine environment proved by previous studies. However, in the P1 site (in center of warm pool of western Pacific), large part of 16S rRNA gene sequences formed a separated cluster. Meanwhile,hzogene sequences from P1 sediment also grouped into a single cluster. PCoA analysis demonstrated that the anammox community structure in the P1 has significant geographical distributional difference from that of P2, P3, and other marine environments based on 16S rRNA andhzogenes. The abundances of anammox bacteria in surface sediments of equatorial Pacific were quantified byq-PCR analysis ofhzogenes, which ranged from 3.98 × 103to 1.17 × 104copies g−1dry sediments. These results suggested that a special anammox bacteria phylotypes exist in the surface sediment of the western Pacific warm pool, which adapted to the specific habitat and maybe involved in the nitrogen loss process from the fixed inventory in the habitat.