AMOUNTS OF MITOCHONDRIAL-DNA AND ABUNDANCE OF SOME MITOCHONDRIAL GENE TRANSCRIPTS IN EARLY MOUSE EMBRYOS
AMOUNTS OF MITOCHONDRIAL-DNA AND ABUNDANCE OF SOME MITOCHONDRIAL GENE TRANSCRIPTS IN EARLY MOUSE EMBRYOS
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DOI：
10.1016/0012-1606(87)90395-2
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发表时间：
1987-10-01
影响因子：
2.7
通讯作者：
TAYLOR, KD
中科院分区：
文献类型：
--
作者：
PIKO, L;TAYLOR, KD
以克隆的线粒体DNA片段为探针，用斑点杂交法测定了从单细胞到囊胚发育过程中线粒体DNA（mtDNA）含量、12和16 S线粒体rRNA以及细胞色素c氧化酶亚基I和II（COI和COII）mRNAs的稳态含量。在此期间，mtDNA含量保持恒定，约为2.13 pg或每个胚胎119，000个mtDNA分子，表明mtDNA复制的缺乏。线粒体rRNA和COI和COII的mRNA的量根据发育阶段而显著变化。它们在卵母细胞生长结束和二细胞期晚期之间保持低水平，但增加了25-50倍。在从两细胞分裂到早期胚泡期间。在早期胚泡中，线粒体mRNA分子的数目估计为7.9 × 104。106或约23%的总胚胎poly（A）+ RNA。这些结果表明，线粒体基因组在很大程度上是不活跃的鸡蛋和两个细胞胚胎，但高速率的线粒体转录在卵裂过程中启动。线粒体基因组的激活与线粒体的显著结构和功能分化相一致。
The contents of mitochondrial DNA (mtDNA) and the steady-state amounts of 12 and 16 S mitochondrial rRNAs and the mRNAs for cytochrome c oxidase subunits I and II (COI and COII) were determined in dot hybridization experiments with cloned mtDNA fragments as probes during development from the one-cell to the blastocyst stage. The mtDNA content remained constant during this period at about 2.13 pg or 119,000 mtDNA molecules per embryos, suggesting an absence of mtDNA replication. The amounts of mitochondrial rRNA and the mRNAs for COI and COII varied markedly depending on developmental stage. They remained low between the end of oocyte growth and the late two-cell stage but increased 25-50 .times. during cleavage from two-cell to early blastocyst. In the early blastocyst, the number of mitochondrial mRNA molecules was estimated at 7.9 .times. 106 or about 23% of the total embryo poly(A)+ RNA. These results suggest that the mitochondrial genome is largely inactive in the egg and two-cell embryo but that a high rate of mitochondrial transcription is initiated during cleavage. The activation of the mitochondrial genome coincides with a pronounced structural and functional differentiation of the mitochondria.