Hyaluronan in the bovine oviduct—modulation of synthases and receptors during the estrous cycle
Hyaluronan in the bovine oviduct—modulation of synthases and receptors during the estrous cycle
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牛输卵管中的透明质酸——动情周期中合成酶和受体的调节
DOI：
10.1016/j.mce.2003.12.002
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发表时间：
2004
影响因子：
4.1
通讯作者：
R. Einspanier
中科院分区：
文献类型：
--
作者：
S. Ulbrich;M. Schoenfelder;S. Thoene;R. Einspanier
细胞外基质（ECM）成分透明质酸（HA）被认为有助于配子和胚胎的最佳发育。在这项研究中，HA，其酶（HAS 1-3）和受体CD 44，RHAMM，和推定的HARE的调控和定位在牛输卵管在发情周期进行了研究。透明质酸可在整个输卵管固有层中显示。除固有层外，在肌肉层中发现免疫反应性CD 44。至于HA，腔上皮缺乏CD 44。引入实时荧光定量RT-PCR，CD 44发生了显著的周期性变化。此外，CD 44的表达在峡部比壶腹部高得多。RHAMM转录升高，特别是在壶腹在发情期。牛推定的HARE编码序列的主要部分进行了表征。组织分布的分析显示，转录不限于肝，脾，淋巴结，提供了证据，当地假定的HARE介导的营业额的HA。这些结果表明，在牛输卵管的细胞表面受体CD 44，特别是可能反向调节HA在发情周期。HA通过过氧化物酶和受体的局部周转可能表明其参与牛输卵管支持配子成熟和早期胚胎发育。
The extracellular matrix (ECM) component hyaluronan (HA) is considered to contribute to the optimal development of the gametes and the embryo. In this study, the regulation and localization of HA, its synthases (HAS 1–3) and the receptors CD44, RHAMM, and putative HARE were investigated in bovine oviducts during the estrous cycle. HA could be demonstrated in the entire lamina propria of the oviduct. Immunoreactive CD44 was found in the muscle layer additionally to the lamina propria. As for HA the luminal epithelium was devoid of CD44. Introducing quantitative real-time RT–PCR, a remarkable cyclic change of CD44 occurred. In addition, CD44 expression was much higher in the isthmus than in the ampulla. RHAMM transcripts were elevated particularly in the ampulla during estrus. A major part of the bovine putative HARE coding sequence was characterized. An analysis of the tissue distribution revealed transcripts not restricted to liver, spleen, and lymph node, providing evidence for a local putative HARE-mediated turnover of HA. These results suggest that in the bovine oviduct the cell surface receptor CD44 in particular might inversely regulate HA during the estrous cycle. The local turnover of HA through synthases and receptors may indicate its participation in the bovine oviduct supporting gamete maturation and early embryonic development.
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大鼠 175-kDa 透明质酸受体内吞作用的分子克隆和功能表达。
影响因子：
3.3
作者：
Zhou,Bin;Weigel,JanetA;Saxena,Amit;Weigel,PaulH
通讯作者：
Weigel,PaulH
影响因子：
3.7
作者：
Aguiar, DJ;Knudson, W;Knudson, CB
通讯作者：
Knudson, CB
透明质酸在骨骺生长板中的分布：表达 CD44 的骨祖细胞的周转。
影响因子：
4
作者：
Pavasant,P;Shizari,TM;Underhill,CB
通讯作者：
Underhill,CB
内吞作用透明质酸受体 (HARE) 不是 CD44 或 CD54 (ICAM-1)。
DOI：
10.1016/s0006-291x(02)00558-2
发表时间：
2002
影响因子：
3.1
作者：
Weigel,JanetA;Raymond,RobertC;Weigel,PaulH
通讯作者：
Weigel,PaulH
影响因子：
2.7
作者：
Duan Y;Yao P;Du Y;Feng J;Wu Q;Zhu D
通讯作者：
Zhu D