Polyethylenimine-Induced Alterations of Red Blood Cells and Their Recognition by the Complement System and Macrophages.
Polyethylenimine-Induced Alterations of Red Blood Cells and Their Recognition by the Complement System and Macrophages.
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DOI：
10.1021/ab500128q
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发表时间：
2015-02
影响因子：
5.8
通讯作者：
Yi Zhang;Changyong Wang;Rushan Hu;Zong-hua Liu;Wei Xue
中科院分区：
文献类型：
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作者：
Yi Zhang;Changyong Wang;Rushan Hu;Zong-hua Liu;Wei Xue
在实际应用中，生物医学材料引入体内可能会与各种宿主细胞和/或生物大分子相互作用并改变其生理特性。生物材料改变的细胞和/或生物大分子可被宿主免疫系统识别为“非自身”，并可因此引起进一步的免疫应答。在本工作中，基因载体材料支化聚乙烯亚胺（1.8 kDa）（BPEI-1.8k）诱导人红细胞（RBC）的一系列改变，如形态从双凹面盘到球形棘细胞的转变，囊泡形成，尺寸减小，表面电荷从负到正的变化，细胞密度降低，膜氧化和PS外化。此外，BPEI-1. 8 k处理的RBC引起自体补体激活并被自体巨噬细胞识别。这意味着生物医学材料BPEI-1.8k改变了RBC的身份，导致其被自体免疫系统识别。本研究为生物医用材料的生物相容性评价和临床应用提供了新的思路。
In practical applications, biomedical materials introduced in vivo may interact with various host cells and/or biomacromolecules and alter their physiological characteristics. Biomaterial-altered cells and/or biomacromolecules may be recognized as "non-self" by the host immune system and may consequently cause further immune responses. In the present work, the gene carrier material branched polyethylenimine (1.8 kDa) (BPEI-1.8k) induced a series of alterations of human red blood cells (RBCs), such as a morphological transition from biconcave disks to spheroechinocytes, vesiculation, a size decrease, a change in surface charge from negative to positive, a cell density reduction, membrane oxidation, and PS externalization. Furthermore, BPEI-1.8k-treated RBCs caused autologous complement activation and were recognized by autologous macrophages. This implies that the biomedical material BPEI-1.8k changed the identity of the RBCs, leading to their recognition by the autologous immune system. This study provides novel insights for the biocompatibility evaluation and clinical application of biomedical materials.