Increased levels of urokinase plasminogen activator receptor in prostate cancer cells derived from repeated metastasis
Increased levels of urokinase plasminogen activator receptor in prostate cancer cells derived from repeated metastasis
复制标题
DOI：
10.1007/s00345-003-0395-3
复制
发表时间：
2004-04-01
影响因子：
3.4
通讯作者：
Sehgal, I
中科院分区：
文献类型：
--
作者：
Forbes, K;Gillette, K;Sehgal, I
为了了解在逐渐转移的前列腺癌细胞中可能发生的尿激酶系统的改变，我们评估了尿激酶纤溶酶原激活剂受体（uPAR）的表达，体外对玻璃体粘连蛋白的运动，尿激酶纤溶酶原激活剂（uPA）诱导的生长和生长因子对三种细胞系（pc -3和两种继发性转移的衍生物，PC-3M和PC-3MM2）中uPAR表达的调节。DU-145和su- pr1细胞被纳入比较目的。在这些细胞系中，uPAR的表达随着转移通道的增加而增加，并伴随着uPA存在下的生长和运动反应的增加。生长因子TGFbeta1和IGF-1诱导三种前列腺癌细胞系的uPAR；然而，PC-3M和PC-3MM2细胞也对bFGF有反应。在测试的细胞系中，PC-3MM2对添加TGFbeta1、IGF-1和bFGF的反应最为均匀。这些结果表明，在PC-3细胞重复转移的两种进行性衍生物中，组成型和生长因子诱导的uPAR表达增强。这种增加的uPAR促进了生长和运动的特性。
To understand alterations to the urokinase system that may occur in progressively metastatic prostate cancer cells, we assessed urokinase plasminogen activator receptor (uPAR) expression, in vitro motility towards vitronectin, urokinase plasminogen activator (uPA)-induced growth and growth factor regulation of uPAR expression in three cell lines-PC-3 and two derivatives from secondary metastases, PC-3M and PC-3MM2. DU-145 and Tsu-Pr1 cells were included for comparative purposes. uPAR expression increases with metastatic passage in these cell lines and accompanies increased growth and motility responses in the presence of uPA. Growth factors TGFbeta1 and IGF-1 induce uPAR in all three prostate cancer lines; however, PC-3M and PC-3MM2 cells also respond to bFGF. Of the cell lines tested, PC-3MM2 most uniformly respond to added TGFbeta1, IGF-1 and bFGF. These results show that in two progressive derivatives from repeated metastasis of PC-3 cells, constitutive and growth factor-induced uPAR expression is enhanced. This increased uPAR facilitates the properties of growth and motility.