Automated Enzymatic Mitochondrial Antibody Assay for the Diagnosis of Primary Biliary Cirrhosis
Automated Enzymatic Mitochondrial Antibody Assay for the Diagnosis of Primary Biliary Cirrhosis
复制标题
用于诊断原发性胆汁性肝硬化的自动酶促线粒体抗体测定
DOI：
10.1515/cclm.2000.107
复制
发表时间：
2000
影响因子：
4.6
通讯作者：
I. Mackay
中科院分区：
文献类型：
--
作者：
W. A. Jensen;J. Jois;P. Murphy;Joseph De Giorgio;Belinda Brown;M. Rowley;I. Mackay
摘要原发性胆汁性肝硬变是一种进行性自身免疫性疾病，影响中年女性，最终导致肝硬变。我们在这里描述了一种自动化的酶线粒体抗体检测方法，适用于用于原发性胆汁性肝硬变的血清学诊断的实验室分析仪。本试验检测针对丙酮酸脱氢酶复合体74 kDa E2亚基的特异性自身抗体。受试者操作员特征曲线数据分析表明，自动酶促线粒体分析方法可将临床确诊的原发性胆汁性肝硬变患者与正常人区分开来，其敏感性为83%，特异性为100%。该方法的灵敏度为73%，特异度为100%，优于商品化的ELISA法。自动化酶线粒体抗体检测是一种高通量检测方法，可用于丙酮酸脱氢酶复合体E2亚单位自身抗体阳性的原发性胆汁性肝硬变患者的常规诊断。该方法对在人群中的高危人群，即中年妇女，进行无症状的原发性胆汁性肝硬变的经济和快速筛查具有潜在的价值。
Abstract Primary biliary cirrhosis is a progressive autoimmune disease that affects middle aged women, resulting in liver cirrhosis. We describe here an automated enzymatic mitochondrial antibody assay adapted for performance on laboratory analysers for the serological diagnosis of primary biliary cirrhosis. This assay detects the characteristic autoantibody directed against the 74kDa E2 subunit of the pyruvate dehydrogenase complex. Analysis of receiver operator characteristic curve data indicated that the automated enzymatic mitochondrial assay procedure discriminated clinically identified patients with primary biliary cirrhosis from normal subjects with a sensitivity of 83% and a specificity of 100%. This method compared favourably against a commercial ELISA method which had a sensitivity of 73% and a specificity of 100%. The automated enzymatic mitochondrial antibody assay is a high throughput assay of use for the routine diagnosis of patients with primary biliary cirrhosis with autoantibodies to the E2 subunit of the pyruvate dehydrogenase complex. The method is of potential value for economical and rapid screening to detect asymptomatic primary biliary cirrhosis in the at-risk segment of the population, namely middle aged women.
影响因子：
158.5
作者：
VANDEWATER, J;COOPER, A;GERSHWIN, ME
通讯作者：
GERSHWIN, ME
原发性胆汁性肝硬化：自身免疫的范例或悖论。
影响因子：
29.4
作者：
Gershwin,ME;Mackay,IR
通讯作者：
Mackay,IR