Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit.
Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit.
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DOI：
10.3389/fmicb.2021.714508
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发表时间：
2021
影响因子：
5.2
通讯作者：
Butt JN
中科院分区：
文献类型：
--
作者：
Piper SEH;Edwards MJ;van Wonderen JH;Casadevall C;Martel A;Jeuken LJC;Reisner E;Clarke TA;Butt JN
希瓦氏菌在细胞代谢和外部氧化还原伙伴之间交换电子，这一过程在绿色电力（微生物燃料电池）和化学品（微生物电合成）的生产中引起了广泛关注。该途径的一个关键组成部分是跨越MTR复合物的外膜，这是由MtrA、MtrB和MtrC蛋白组成的生物分子线。MtrA和MtrC是十血红素细胞色素，它们形成一条紧密排列的血红素链，定义了185 Å的电子传递途径。MtrA包裹在MtrB内部，以便在外膜脂双分子层上溶解；MtrC位于细胞外，与外部氧化还原伙伴进行电子交换。在这里，我们证明了MtrAB与单独纯化的MtrC在体外紧密自发的关联。所得到的复合物在结构和跨脂质双分子层的电子转移速率方面与希瓦氏菌自然组装的MTR复合物相当。我们的研究结果揭示了构建定制电子管道的潜力，其中MtrAB与化学修饰的MtrC结合，在这种情况下，用ru染料标记，使光触发电子注入MtrC血红素链。
Shewanella oneidensis exchanges electrons between cellular metabolism and external redox partners in a process that attracts much attention for production of green electricity (microbial fuel cells) and chemicals (microbial electrosynthesis). A critical component of this pathway is the outer membrane spanning MTR complex, a biomolecular wire formed of the MtrA, MtrB, and MtrC proteins. MtrA and MtrC are decaheme cytochromes that form a chain of close-packed hemes to define an electron transfer pathway of 185 Å. MtrA is wrapped inside MtrB for solubility across the outer membrane lipid bilayer; MtrC sits outside the cell for electron exchange with external redox partners. Here, we demonstrate tight and spontaneous in vitro association of MtrAB with separately purified MtrC. The resulting complex is comparable with the MTR complex naturally assembled by Shewanella in terms of both its structure and rates of electron transfer across a lipid bilayer. Our findings reveal the potential for building bespoke electron conduits where MtrAB combines with chemically modified MtrC, in this case, labeled with a Ru-dye that enables light-triggered electron injection into the MtrC heme chain.