Demonstration of asymmetric electron conduction in pseudosymmetrical photosynthetic reaction centre proteins in an electrical circuit.
Demonstration of asymmetric electron conduction in pseudosymmetrical photosynthetic reaction centre proteins in an electrical circuit.
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DOI：
10.1038/ncomms7530
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发表时间：
2015-03-09
影响因子：
16.6
通讯作者：
Jones MR
中科院分区：
文献类型：
--
作者：
Kamran M;Friebe VM;Delgado JD;Aartsma TJ;Frese RN;Jones MR
光合作用反应中心显示出生物分子电子学的前景，如纳米级太阳能电池和分子二极管，它们可以进行原子级的重新设计。在这项工作中，电子传导机制的高度听话的Rhodobacter sphaeroides反应中心的特点是导电原子力显微镜。我们发现，使用已知结构的工程蛋白质，只有一个连接该反应中心的正和负末端的两个辅因子线能够在适当取向的偏压下传导单向电流，而不管偏压的大小或在隧道结处施加的力。这种行为，在很大程度上是对称的蛋白质辅因子矩阵中的强功能不对称性，概括了天然光化学电荷分离的强功能不对称性特征，但令人惊讶的是，电子流的刺激仅仅是外部施加的偏压。探讨了辅因子的所谓B线所显示的电阻的原因。 光合反应中心已被提出用于生物电子学中的应用。在这里，作者研究了电子通过反应中心从R。使用导电AFM测量球形晶体，观察在施加偏压下仅沿一条辅因子线的沿着不对称电导。
Photosynthetic reaction centres show promise for biomolecular electronics as nanoscale solar-powered batteries and molecular diodes that are amenable to atomic-level re-engineering. In this work the mechanism of electron conduction across the highly tractable Rhodobacter sphaeroides reaction centre is characterized by conductive atomic force microscopy. We find, using engineered proteins of known structure, that only one of the two cofactor wires connecting the positive and negative termini of this reaction centre is capable of conducting unidirectional current under a suitably oriented bias, irrespective of the magnitude of the bias or the applied force at the tunnelling junction. This behaviour, strong functional asymmetry in a largely symmetrical protein–cofactor matrix, recapitulates the strong functional asymmetry characteristic of natural photochemical charge separation, but it is surprising given that the stimulus for electron flow is simply an externally applied bias. Reasons for the electrical resistance displayed by the so-called B-wire of cofactors are explored. Photosynthetic reaction centres have been proposed for applications in bioelectronics. Here, the authors examine electron transport through the reaction centre from R. sphaeroides using conductive AFM, observing asymmetric conductance along only one cofactor wire under an applied bias.
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