Fluorescence analysis of size distribution and mode of dye release from carboxyfluorescein-loaded vesicles: application to the study of complement-membrane interactions.
Fluorescence analysis of size distribution and mode of dye release from carboxyfluorescein-loaded vesicles: application to the study of complement-membrane interactions.
复制标题
负载羧基荧光素的囊泡的尺寸分布和染料释放模式的荧光分析：在补体膜相互作用研究中的应用。
DOI：
10.1016/0005-2736(88)90487-7
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hu,VW
中科院分区：
文献类型：
--
作者：
Liu,ZY;Solow,R;Hu,VW
We wish to report a novel method for visualizing large unilamellar vesicles loaded with a fluorescent dye and for monitoring changes in the size distribution as well as state of aggregation of such dye-loaded liposomes. In addition, we demonstrate that this method can be used to distinguish between all-or-none release of dye and graded release of dye from individual vesicles. Using this technique, we have characterized complement-mediated release of car☐yfluorescein from large unilamellar vesicles and have found that C5–8 complexes mediate a graded release of dye while C5–9 complexes cause an all-or-none release. Furthermore, complement appears to preferentially attack the medium to larger-sized vesicles in our population of large unilamellar vesicles while smaller vesicles appear to be selectively spared.
