Multiresolution Localization With Temporal Scanning for Super-Resolution Diffuse Optical Imaging of Fluorescence
Multiresolution Localization With Temporal Scanning for Super-Resolution Diffuse Optical Imaging of Fluorescence
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DOI：
10.1109/tip.2019.2931080
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发表时间：
2020-01-01
影响因子：
10.6
通讯作者：
Webb，Kevin J.
中科院分区：
文献类型：
--
作者：
Bentz，Brian Z.;Lin，Dergan;Webb，Kevin J.
提出了一种超分辨率光学成像方法，该方法依赖于与每个荧光光学报告物相关联的不同的时间信息，以通过测量严重散射的光来高精度地确定其空间位置。这种多发射器定位方法在成本函数中使用扩散方程前向模型，并且具有通过厘米组织实现微米尺度空间分辨率的潜力。利用一定程度的时间分离的报告发射，位置和发射强度确定使用计算高效的时间扫描多分辨率算法。该方法通过使用扩散方程前向模型来规避早期光学成像方法所面临的空间分辨率挑战，并且有希望用于体内应用。例如，原则上，该方法可用于定位整个啮齿动物大脑中的单个神经元放电，从而实现神经网络活动的直接成像。
A super-resolution optical imaging method is presented that relies on the distinct temporal information associated with each fluorescent optical reporter to determine its spatial position to high precision with measurements of heavily scattered light. This multiple-emitter localization approach uses a diffusion equation forward model in a cost function, and has the potential to achieve micron-scale spatial resolution through centimeters of tissue. Utilizing some degree of temporal separation for the reporter emissions, position and emission strength are determined using a computationally efficient temporal-scanning multiresolution algorithm. The approach circumvents the spatial resolution challenges faced by earlier optical imaging approaches by using a diffusion equation forward model, and is promising for in vivo applications. For example, in principle, the method could be used to localize individual neurons firing throughout a rodent brain, enabling the direct imaging of neural network activity.