Relationship of anticardiolipin antibodies and antiphospholipid syndrome to HLA-DR7 in Mexican patients with systemic lupus erythematosus (SLE)
Relationship of anticardiolipin antibodies and antiphospholipid syndrome to HLA-DR7 in Mexican patients with systemic lupus erythematosus (SLE)
复制标题
墨西哥系统性红斑狼疮 (SLE) 患者抗心磷脂抗体和抗磷脂综合征与 HLA-DR7 的关系
作者：
J. Granados;G. Vargas;C. Drenkard;F. Andrade;H. Melín‐Aldana;J. Alcocer;D. Alarcon
我们研究了IgG和IgM抗心磷脂抗体（ACL）的ELISA方法在80例墨西哥系统性红斑狼疮（SLE）患者和378他们的一级亲属。65%的SLE患者和16%的亲属aCL阳性。我们还确定了患者和亲属的主要组织相容性复合体（MHC）基因（I类，II类和III类）的等位基因和单倍型频率。将aCL阳性和阴性个体的MHC等位基因和单倍型频率与正常种族匹配的对照组进行比较。SLE合并aCL患者HLA-DR 3（pC = 0.04，RR = 2.78）、DR 7（pC = 0.005，RR = 3.42）和DQ 2（pC = 0.003，RR = 2.58）抗原的校正频率显著增加。aCL阳性的一级亲属HLA-DR 7的频率也有增加，但校正P值后没有保持显著性。另一方面，aCL阴性的SLE患者DR 3和DQ 2的频率中度增加，但DR 7的频率没有增加。这些结果表明DR 7与aCL的存在相关。aCL阳性SLE患者MHC等位基因的分布与aCL阳性一级亲属相似。由于抗体的存在不足以预测临床结果，我们研究了那些有可靠临床数据的抗磷脂综合征（aPLS）患者。aPLS的SLE患者DR 7的频率显著增加（pC = 0.004），可能aPLS的患者DR 7的频率也显著增加（pC = 0.05），而可疑或阴性aPLS类别的SLE患者DR 7的频率与正常对照无差异。这些数据支持DR 7在SLE患者及其亲属的aCL发展中的可能作用，并表明这种II类MHC抗原对SLE内aPLS的发展有贡献。
We studied IgG and IgM anticardiolipin antibodies (aCL) by an ELISA method in 80 Mexican systemic lupus erythematosus (SLE) patients and 378 of their first degree relatives. Sixty five percent of SLE patients and 16% of their relatives were positive for aCL. We also determined allele and haplotype frequencies of Major Histocompatibility Complex (MHC) genes (classes I, II and III) in both patients and relatives. MHC allele and haplotype frequencies of aCL positive and negative individuals were compared to those of normal ethnically matched controls. SLE patients with aCL had statistically significant increased corrected frequencies of HLA-DR3 (pC = 0.04, RR = 2.78); DR7 (pC = 0.005), RR = 3.42) and DQ2 (pC = 0.003, RR = 2.58) antigens. Their first degree relatives positive for aCL also had increased frequency of HLA-DR7 but it did not remain significant after correcting the P value. On the other hand, SLE patients negative for aCL had a moderate increased frequency of DR3 and DQ2 but not of DR7. These results suggest that DR7 associates with the presence of aCL. The distribution of MHC alleles in SLE patients positive for aCL resembles that found in their aCL positive first degree relatives. Since the presence of the antibody is not sufficient to predict a clinical outcome, we studied those patients with reliable clinical data regarding the presence of the antiphospholipid syndrome (aPLS). SLE patients with aPLS had significantly increased frequency of DR7 (pC = 0.004), as did those with probable aPLS (pC = 0.05), while the frequency of DR7 in SLE patients in the doubtful or negative aPLS categories was no different from normal controls. These data support a possible role of DR7 in the development of aCL in SLE patients and their relatives and suggest a contribution of this class II MHC antigen to the development of aPLS within SLE.
与狼疮抗凝剂相关的主要组织相容性复杂等位基因的分子分析。
DOI：
10.1172/jci115158
发表时间：
1991
期刊：
The Journal of clinical investigation
影响因子：
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作者：
Arnett,FC;Olsen,ML;Anderson,KL;Reveille,JD
通讯作者：
Reveille,JD