In vivo tissue engineering of a trilayered leaflet-shaped tissue construct.
In vivo tissue engineering of a trilayered leaflet-shaped tissue construct.
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DOI：
10.2217/rme-2019-0078
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发表时间：
2020-01
影响因子：
2.7
通讯作者：
S. Jana;A. Lerman
中科院分区：
文献类型：
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作者：
S. Jana;A. Lerman
目的：我们旨在开发一种小叶形三层组织结构，模仿天然心脏瓣膜小叶的形态。材料与方法：采用静电纺丝技术和体内组织工程方法。结果如下：我们开发了小叶形微纤维支架，每个支架具有周向、随机和径向定向的三层，模仿天然小叶的三层定向结构。在使用支架进行体内组织工程3个月后，生成的小叶形组织构建体具有模仿天然心脏瓣膜小叶的取向的三层结构。在工程化组织构建体中观察到天然小叶中观察到的胶原蛋白、糖胺聚糖和弹性蛋白的存在。结论：三层定向纤维支架将浸润细胞及其产生的细胞外基质蛋白的方向带入构建体中。
Aim: We aimed to develop a leaflet-shaped trilayered tissue construct mimicking the morphology of native heart valve leaflets. Materials & methods: Electrospinning and in vivo tissue engineering methods were employed. Results: We developed leaflet-shaped microfibrous scaffolds, each with circumferentially, randomly and radially oriented three layers mimicking the trilayered, oriented structure of native leaflets. After 3 months in vivo tissue engineering with the scaffolds, the generated leaflet-shaped tissue constructs had a trilayered structure mimicking the orientations of native heart valve leaflets. Presence of collagen, glycosaminoglycans and elastin seen in native leaflets was observed in the engineered tissue constructs. Conclusion: Trilayered, oriented fibrous scaffolds brought the orientations of the infiltrated cells and their produced extracellular matrix proteins into the constructs.