Correlation of TWIST2 up-regulation and epithelial-mesenchymal transition during tumorigenesis and progression of cervical carcinoma
Correlation of TWIST2 up-regulation and epithelial-mesenchymal transition during tumorigenesis and progression of cervical carcinoma
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宫颈癌发生发展过程中TWIST2表达上调与上皮间质转化的相关性
DOI：
10.1016/j.ygyno.2011.09.003
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发表时间：
2012-01-01
影响因子：
4.7
通讯作者：
Wang, Shixuan
中科院分区：
文献类型：
--
作者：
Li, Yan;Wang, Wei;Wang, Shixuan
Objective.在全球范围内，宫颈癌是女性中第二常见的癌症，确定参与肿瘤进展的潜在靶点是必要的。本研究旨在探讨一种碱性螺旋-环-螺旋转录因子twist homolog 2（IWIST 2）在宫颈癌中的表达及其与E-cadherin表达的相关性。采用TWIST 2和E-cadherin免疫组化染色方法检测14例正常宫颈组织、58例宫颈上皮内瘤变（CIN）和70例鳞状细胞癌（SCC）组织中TWIST 2和E-cadherin的表达，并进行统计学分析。TWIST 2在细胞质和细胞核中的表达水平升高与宫颈上皮的恶性转化和宫颈癌的组织学进展有关。Logistic检验显示TWIST 2是SCC淋巴结转移的相对独立的预测因子。此外，TWIST 2水平的增加也与E-cadherin的异常表达有关，E-cadherin是一个重要的EMT指标。目前的数据表明，TWIST 2的过度表达与宫颈癌的进展，这使得它成为一个有前途的标志物，用于确定宫颈癌的转移潜力，和TWIST 2的上调，结合异常E-cadherin表达在原发性宫颈癌组织中，可以预测恶性转化和癌的远端转移。(C)2011 Elsevier Inc. All rights reserved.
Objective. Globally, cervical cancer is the second most common cancer among women, and determining potential targets involved in tumor progression is necessary. This study investigated the clinic-pathological significance of twist homolog 2 (IWIST2), a basic helix-loop-helix transcription factor, and correlated TWIST2 and E-cadherin expression in cervical cancer.Methods. A series of 142 samples, including 14 cases of normal cervical tissues, 58 cases of cervical intraepithelial neoplasia (CIN) and 70 cases of sguamous cell carcinoma (SCC), were examined TWIST2 and E-cadherin immunohistochemical staining and statistical analysis.Results. Increased cytoplasmic and nuclear expression levels of TWIST2 were associated with the malignant transformation of cervical epithelium and the histological progression of cervical cancer. A logistic test showed that TWIST2 was a relatively independent predictor of lymph node metastasis of SCC. Further, increased levels of TWIST2 were also associated with aberrant expression of E-cadherin, an important EMT indicator.Conclusions. The present data suggest that TWIST2 overexpression was significantly linked to cervical cancer progression, which makes it a promising marker for determining the metastatic potential of cervical cancer, and up-regulation of TWIST2, in combination with aberrant E-cadherin expression in primary cervical cancer tissues, may predict the malignant transformation and distal metastasis of carcinomas. (C) 2011 Elsevier Inc. All rights reserved.