Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes
Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes
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DOI：
10.1101/gad.615211
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发表时间：
2011-05-01
影响因子：
10.5
通讯作者：
Gurdon, John B.
中科院分区：
文献类型：
--
作者：
Miyamoto, Kei;Pasque, Vincent;Gurdon, John B.
两栖类卵母细胞能快速有效地重编程移植体细胞核的转录。为了探索相关的因素和机制，我们将重点放在核肌动蛋白上，这是卵母细胞核（生殖囊泡）中特别丰富的成分。核作用的存在和意义一直是争论不休的.在这里，我们发现核肌动蛋白聚合在多能性基因Oct 4（也称为Pou 5 f1）的转录再激活中起着重要作用。我们还发现肌动蛋白信号蛋白Toca-1通过调节核肌动蛋白聚合来增强Oct 4的再活化。Toca-1过表达对移植细胞核的染色质状态有影响，包括核肌动蛋白与基因调控区的结合增强。这是第一份报告表明，在卵母细胞核中自然储存的肌动蛋白有助于转录重编程的聚合依赖的方式。
Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei. To explore the factors and mechanisms involved, we focused on nuclear actin, an especially abundant component of the oocyte's nucleus (the germinal vesicle). The existence and significance of nuclear actin has long been debated. Here, we found that nuclear actin polymerization plays an essential part in the transcriptional reactivation of the pluripotency gene Oct4 (also known as Pou5f1). We also found that an actin signaling protein, Toca-1, enhances Oct4 reactivation by regulating nuclear actin polymerization. Toca-1 overexpression has an effect on the chromatin state of transplanted nuclei, including the enhanced binding of nuclear actin to gene regulatory regions. This is the first report showing that naturally stored actin in an oocyte nucleus helps transcriptional reprogramming in a polymerization-dependent manner.