cDNA cloning of heat shock proteins and their expression in the two phases of the migratory locust
cDNA cloning of heat shock proteins and their expression in the two phases of the migratory locust
复制标题
飞蝗热激蛋白的cDNA克隆及其在两阶段的表达
DOI：
10.1111/j.1365-2583.2006.00715.x
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发表时间：
2007-04-01
影响因子：
2.6
通讯作者：
Kang, L.
中科院分区：
文献类型：
--
作者：
Wang, H. -S.;Wang, X. -H.;Kang, L.
昆虫的高种群密度往往是一个压力因素。昆虫通过分子伴侣活性合成热激蛋白（Hsps）来响应胁迫的影响。蝗虫的独居和群居阶段分别发生在低和高人口密度。本研究比较了Hsp基因在飞蝗两个阶段的表达谱，试图探讨Hsp在飞蝗适应高密度种群中的作用。克隆了飞蝗热休克蛋白20.5、20.6、20.7、40、70和热休克蛋白90的全长cDNA并进行了序列测定。这6种热休克蛋白在群居型蝗虫和独居型蝗虫中的表达存在差异。来自真实的-时间PCR的结果表明，六种Hsps在除了早期胚胎之外的所有发育阶段中表达。这6种热休克蛋白在群居蝗虫中的表达量均显著上调。群居蝗虫5龄幼虫头、胸、腿等器官间的表达差异也高于独居蝗虫。这些观察结果表明，人口密度可能是一个重要的因素，在确定热休克蛋白在蝗虫的表达。
The high population density of insects is often a stress factor. Insects synthesize heat shock proteins (Hsps) in response to the impacts of stress through molecular chaperone activity. Locust solitary and gregarious phases occur at low and high population density, respectively. In this study, we compare the expression profiles of the Hsp genes in the two phases of the migratory locust in an attempt to examine the role of Hsps in adaptation to high density populations. The full length cDNAs of Hsp20.5, 20.6, 20.7, 40, 70 and Hsp90 of the migratory locust were cloned and sequenced. The expressional differentiation of the six Hsps in mRNA levels between solitary and gregarious locusts was observed. Results from real‐time PCR indicate that the six Hsps are expressed throughout all developmental stages except in the early stage embryo. The expression levels of the six Hsps were significantly upregulated in gregarious locusts. The expressional variations among certain organs, such as the head, thorax and leg of fifth instar nymphs in gregarious locusts were also higher than those in solitary ones. These observations suggest that population density may be an important factor in determining Hsp expression in the locust.