Class targeted metabolomics:: ESI ion trap screening methods for glucosinolates based on MSn fragmentation
Class targeted metabolomics:: ESI ion trap screening methods for glucosinolates based on MSn fragmentation
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DOI：
10.1016/j.phytochem.2008.02.010
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发表时间：
2008-05-01
期刊：
影响因子：
3.8
通讯作者：
Jones, Rod
中科院分区：
文献类型：
--
作者：
Rochfort, Simone J.;Trenerry, V. Craige;Jones, Rod
硫代葡萄糖苷是天然存在的阴离子次生植物代谢产物，其在磺化肟的碳上掺入硫代葡萄糖苷键。存在大量天然存在的芥子油苷，并且它们在十字花科的许多植物物种中相对大量地被发现。这些代谢物因其抗癌和风味特性以及在模式植物如拟南芥中的氮和硫代谢的研究中而令人感兴趣。母离子图谱是一种分析质谱法，可以快速评估硫代葡萄糖甙含量。离子图谱被证明是高度敏感的，芥子油苷黑芥子苷可以检测到万亿分之三。该方法利用硫代葡萄糖酸盐阴离子片段化，其在离子阱质谱仪中始终产生磺酸盐开环葡萄糖部分，m/z 259。这种断裂的分子内转移机制在这里首次提出。这种片段化可以用作植物提取物中硫代葡萄糖苷类代谢物的一般标识符，并且在LCMSn中可以用于提供单个硫代葡萄糖苷的阳性鉴定和定量。这些方法提供了敏感的工具，集中代谢组学分析和筛选植物育种系。(C)2008爱思唯尔有限公司保留所有权利。
Glucosinolates are naturally occurring anionic secondary plant metabolites incorporating a thioglucosidic link to the carbon of a sulphonated oxime. There are a large number of naturally occurring glucosinolates and they are found in relatively large quantities in many plant species within the family Crucifereae. These metabolites are of interest for both their anticancer and flavour properties and in the study of nitrogen and sulphur metabolism in model plants such as Arabidopsis. Parent ion mapping is an analytical mass spectrometry approach that allows rapid assessment of glucosinolate content. Ion mapping proved to be highly sensitive and the glucosinolate sinigrin could be detected at three parts per trillion. This method takes advantage of the glucosinolate anion fragmentation which consistently produces a sulphonate ring-opened glucose moiety in the ion trap mass spectrometer, m/z 259. An intramolecular transfer mechanism for this fragmentation is presented here for the first time. This fragmentation can be exploited as a general identifier of the glucosinolate class of metabolites in plant extracts and in LCMSn can be employed provide positive identification and quantification of individual glucosinolates. Such approaches offer sensitive tools for focused metabolomics analysis and screening of plant breeding lines. (C) 2008 Elsevier Ltd. All rights reserved.