Prostaglandin F2α-PTGFR signaling promotes proliferation of endometrial epithelial cells of cattle through cell cycle regulation
Prostaglandin F2α-PTGFR signaling promotes proliferation of endometrial epithelial cells of cattle through cell cycle regulation
复制标题
前列腺素 F2α-PTGFR 信号通过细胞周期调节促进牛子宫内膜上皮细胞的增殖。
DOI：
10.1016/j.anireprosci.2020.106276
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发表时间：
2020-02-01
影响因子：
2.2
通讯作者：
Liu, Bo
中科院分区：
文献类型：
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作者：
Fu, Changqi;Mao, Wei;Liu, Bo
牛子宫内膜上皮细胞中存在前列腺素F-2 α（PGF（2 α））的产生和PGF（2 α）受体（PTGFR）mRNA转录本。本研究的目的是（1）确定PGF（2 α）-PTGFR信号传导是否调节子宫内膜上皮细胞的增殖和（2）增加对牛子宫内膜中生理和药理过程的PGF（2 α-）PTGFR信号传导的了解。使用实时细胞分析和细胞增殖试剂WST-1程序测定细胞增殖量。采用实时荧光定量RT-PCR和Western blot检测细胞周期蛋白、细胞周期蛋白依赖性激酶（CDKs）、细胞周期蛋白激酶抑制剂、增殖细胞核抗原（PCNA）、环氧合酶-1（考克斯-1）、环氧合酶-2（考克斯-2）、PTGFR、表皮生长因子（EGF）mRNA和蛋白的表达。PGF（2 α）-PTGFR信号通过诱导mRNA转录物和蛋白质丰度的变化促进子宫内膜上皮细胞增殖，导致细胞周期蛋白（A、B1、D1、D3）、CDKs（1、2、4、6）和PCNA丰度增加; p21丰度减少; EGF、考克斯-1、考克斯-2和PTGFR丰度增加。在牛子宫内膜上皮细胞中，PGF（2 α）-PTGFR信号传导与细胞周期调控之间存在直接的分子关联。此外，研究结果提高了对牛子宫内膜生理和药理过程中PGF（2 α）-PTGFR信号传导的理解。
There is production of prostaglandin F-2 alpha (PGF(2 alpha)) and there is PGF(2 alpha) receptor (PTGFR) mRNA transcript in endometrial epithelial cells of cattle. The aims of the present study were to (1) determine whether PGF(2 alpha)-PTGFR signaling modulates the proliferation of endometrial epithelial cells and (2) increase knowledge of PGF(2 alpha-)PTGFR signaling on the physiological and pharmacological processes in the endometrium of cattle. Amount of cellular proliferation was determined using real-time cell analysis and cell proliferation reagent WST-1 procedures. Abundance of cyclins, cyclin-dependent kinases (CDKs), cyclin-kinase inhibitors, proliferating cell nuclear antigen (PCNA), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), PTGFR, epidermal growth factor (EGF) mRNA and protein abundances were evaluated using real-time RT-PCR and western blot analyses. The PGF(2 alpha)-PTGFR signaling promoted the proliferation of endometrial epithelial cells by inducing changes in abundance of mRNA transcript and protein that resulted in an increase in the abundance for the cyclins (A, B1, D1, D3), CDKs (1, 2, 4, 6), and PCNA; decrease in abundance for p21; and increase in abundance for EGF, COX-1, COX-2, and PTGFR. There was a direct molecular association between PGF(2 alpha)-PTGFR signaling and cell cycle regulation in endometrial epithelial cells of cattle. In addition, findings improve the understanding of PGF(2 alpha)-PTGFR signaling in the physiological and pharmacological processes of the endometrium of cattle.