Eccentric Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models to Impair Skeletal Muscle's Capacity to Generate Force
Eccentric Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models to Impair Skeletal Muscle's Capacity to Generate Force
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DOI：
10.1007/978-1-4939-3810-0_1
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Lowe, Dawn A.
中科院分区：
文献类型：
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作者：
Call, Jarrod A.;Lowe, Dawn A.
为了研究肌肉再生的分子和细胞机制，需要实验损伤模型。偏心收缩性损伤的优势在于它是一种可控的、可重复的、与生理相关的肌肉损伤模型，损伤被定义为丧失发力能力。虽然离心收缩可以被纳入有意识的动物研究设计中，如下坡跑步机，但电生理学方法可以引起离心收缩并检查肌肉收缩力，例如在有害的离心收缩之前和之后，使研究人员能够规避确定有意识动物肌肉功能的常见问题（例如，不愿意参与）。在这里，我们描述了可靠的、可重复的、真正最大的体外和体内方法，因为肌肉收缩是在受控的、可量化的方式下被激发的，独立于受试者的动机。这两种方法都可用于引发偏心收缩性损伤，并适用于损伤后数小时至数天至数周的功能性肌肉再生监测。
In order to investigate the molecular and cellular mechanisms of muscle regeneration an experimental injury model is required. Advantages of eccentric contraction-induced injury are that it is a controllable, reproducible, and physiologically relevant model to cause muscle injury, with injury being defined as a loss of force generating capacity. While eccentric contractions can be incorporated into conscious animal study designs such as downhill treadmill running, electrophysiological approaches to elicit eccentric contractions and examine muscle contractility, for example before and after the injurious eccentric contractions, allows researchers to circumvent common issues in determining muscle function in a conscious animal (e.g., unwillingness to participate). Herein, we describe in vitro and in vivo methods that are reliable, repeatable, and truly maximal because the muscle contractions are evoked in a controlled, quantifiable manner independent of subject motivation. Both methods can be used to initiate eccentric contraction-induced injury and are suitable for monitoring functional muscle regeneration hours to days to weeks post-injury.